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CTCF Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33854

Novus Biologicals, part of Bio-Techne
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NBP2-33854
NBP2-33854-25ul

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: QNQTDGGEVVQDVNSSVQMVMMEQLDPTLLQMKTEVMEGTVAPEAEAAVDDTQIITLQVVNMEEQPINIGELQLVQVPVPVTVPVATTSVEELQGAYENEVSKEGLAESEPMICHTLPLPEGFQVVKVGANGEVETLEQGELPPQEDP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CTCF Antibody

Immunocytochemistry/ Immunofluorescence: CTCF Antibody [NBP2-33854]

Immunocytochemistry/ Immunofluorescence: CTCF Antibody [NBP2-33854]

Immunocytochemistry/Immunofluorescence: CTCF Antibody [NBP2-33854] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
Immunohistochemistry: CTCF Antibody [NBP2-33854]

Immunohistochemistry: CTCF Antibody [NBP2-33854]

Immunohistochemistry: CTCF Antibody [NBP2-33854] - Staining of human colon shows strong nuclear and moderate cytoplasmic positivity in glandular cells.

Applications for CTCF Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CTCF

The CTCF gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA tar

Long Name

CCCTC-binding Factor

Alternate Names

11-Zinc Finger Protein2, CCCTC-binding Factor

Gene Symbol

CTCF

UniProt

Additional CTCF Products

Product Documents for CTCF Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CTCF Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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