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CTGF/CCN2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-66426

Novus Biologicals, part of Bio-Techne
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NBP2-66426

Key Product Details

Species Reactivity

Mouse, Rat

Applications

Control, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-SLYYRKMYGDMA, from the C Terminus of the protein sequence according to NP_001892.

Reactivity Notes

Expected from sequence similarity: Human, Rat and Canine

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for CTGF/CCN2 Antibody

Immunocytochemistry/ Immunofluorescence: CTGF/CCN2 Antibody [NBP2-66426]

Immunocytochemistry/ Immunofluorescence: CTGF/CCN2 Antibody [NBP2-66426]

Immunocytochemistry/Immunofluorescence: CTGF/CCN2 Antibody [NBP2-66426] - Paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (5 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunohistochemistry: CTGF/CCN2 Antibody [NBP2-66426]

Immunohistochemistry: CTGF/CCN2 Antibody [NBP2-66426]

Immunohistochemistry: CTGF/CCN2 Antibody [NBP2-66426] - (6ug/ml) staining of paraffin embedded Rat Liver. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Control: CTGF/CCN2 Antibody [NBP2-66426] - Negative Control showing staining of paraffin embedded Rat Liver, with no primary antibody.

Applications for CTGF/CCN2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

5 ug/mL

Immunohistochemistry

6.0 - 8.0 ug/ml

Peptide ELISA

Detection limit 1:10000
Application Notes
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of NIH3T3 cells.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CTGF/CCN2

Connective Tissue Growth Factor (CTGF) is a 38kDa, cysteine-rich, secreted peptide. CTGF promotes endothelial cell growth, migration, adhesion and survival. and is thus implicated in endothelial cell function and angiogenesis. CTGF is implicated in are embryogenesis, wound healing and regulation of extracellular matrix production, and is required for normal skeletal growth.

CTGF antibodies are useful tools for angiogenesis and cell structure research, and for studies on certian types of cancer.

Long Name

Connective Tissue Growth Factor

Alternate Names

CCN2, CTGRP, Fisp12, HCS24, IGFBP-8, NOV2

Gene Symbol

CCN2

Additional CTGF/CCN2 Products

Product Documents for CTGF/CCN2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CTGF/CCN2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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