CtIP Antibody (19E8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-41205

Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Proximity Ligation Assay, Western Blot

Cited:

Chemotaxis, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 19E8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CtIP Antibodies »

Product Specifications

Immunogen

Human CtIP

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody will recognize human CtIP.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

125 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CtIP Antibody (19E8)

Immunocytochemistry/ Immunofluorescence: CtIP Antibody (19E8) [NBP1-41205]

Immunocytochemistry/ Immunofluorescence: CtIP Antibody (19E8) [NBP1-41205]

Immunocytochemistry/Immunofluorescence: CtIP Antibody (19E8) [NBP1-41205] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CtIP protein stained by CtIP antibody [19E8] diluted at 1:100. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
CtIP Antibody (19E8)

Western Blot: CtIP Antibody (19E8) [NBP1-41205] -

Western Blot: CtIP Antibody (19E8) [NBP1-41205] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CtIP antibody [19E8] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Applications for CtIP Antibody (19E8)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:1000
Application Notes
ChIP and IP usages reported in scientific literature. WB 3% Milk/TBST overnight. IP, Chip, PLA Assay dependent.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CtIP

CtIP, a 125 kD protein, was originally found interacting with a transcription repressor, CtBP, through the PLDLS motif (CtBP-Interacting Protein) thus suggested a role in transcription. Studies have shown that CtIP also interacts with BRCA1 protein through the c-terminus BRCT domains also suggested that CtIP is a potential tumor suppressor. This CtIP-BRCA1 interaction can be disruppted by DNA damaging agents including UV or gamma-irradiation. Li et al (Nature 406, 210 - 215 (2000)) have shown that ATM phosphorylates CtIP at serine residues 664 and 745, and mutation of these sites abrogates the dissociation of BRCA1 from CtIP, resulting in persistent repression of BRCA1-dependent induction of GADD45 upon ionizing radiation.

Alternate Names

CtBP-interacting protein, CtIP, DNA endonuclease RBBP8, EC 3.1, RBBP-8, retinoblastoma binding protein 8, Retinoblastoma-binding protein 8, Retinoblastoma-interacting protein and myosin-like, RIMSAE2, Sporulation in the absence of SPO11 protein 2 homolog

Entrez Gene IDs

5932 (Human)

Gene Symbol

RBBP8

Additional CtIP Products

Product Documents for CtIP Antibody (19E8)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CtIP Antibody (19E8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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