CUGBP1/CELF1 Antibody (3B1)

Novus Biologicals, part of Bio-Techne | Catalog # NB200-316

Novus Biologicals, part of Bio-Techne
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NB200-316
NB200-316SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Primate, Rabbit

Cited:

Human, Mouse, Rat, Primate

Applications

Validated:

Flow Cytometry, Gel Super Shift Assays, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot

Cited:

EMSA, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3B1

Concentration

1 mg/ml

View all CUGBP1/CELF1 Antibodies »

Product Specifications

Immunogen

Full-length human CUGBP1 [UniProt# Q92879]

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CUGBP1/CELF1 Antibody (3B1)

Western Blot: CUGBP1/CELF1 Antibody (3B1) [NB200-316]

Western Blot: CUGBP1/CELF1 Antibody (3B1) [NB200-316]

Western Blot: CUGBP1 Antibody (3B1) [NB200-316] - Detection of CUG-BP1 in several cell lysates.
Immunocytochemistry/ Immunofluorescence: CUGBP1/CELF1 Antibody (3B1) [NB200-316]

Immunocytochemistry/ Immunofluorescence: CUGBP1/CELF1 Antibody (3B1) [NB200-316]

Immunocytochemistry/Immunofluorescence: CUGBP1 Antibody (3B1) [NB200-316] - Detection of the subcellular distribution of CUGBP1 (nuclear, non-nucleolar) in normal and DM1 (dystrophia myotonica) myoblasts.
Immunohistochemistry-Paraffin: CUGBP1/CELF1 Antibody (3B1) [NB200-316]

Immunohistochemistry-Paraffin: CUGBP1/CELF1 Antibody (3B1) [NB200-316]

Immunohistochemistry-Paraffin: CUGBP1/CELF1 Antibody (3B1) [NB200-316] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human spleen using 1:100 conc. of CUGBP1/CELF1 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 30 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 9.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 15 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Nuclear staining was observed in lymphocytes.
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Applications for CUGBP1/CELF1 Antibody (3B1)

Application
Recommended Usage

Flow Cytometry

1 ug per million cells

Gel Super Shift Assays

reported in scientific literature

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Frozen

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

reported in scientific literature

Simple Western

1:200

Western Blot

1:500
Application Notes
In Western Blot, a band is observed at approx. 50 kDa. Nuclear staining can be seen in ICC/IF.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Tris-Glycine and 0.15M NaCl

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CUGBP1/CELF1

CUGBP1 (CUG-binding protein 1) is a member of CELF (CUG-BP- and ETR-3-like factors) family of RNA-binding proteins that binds to a variety of mRNA cis-elements including UG-rich elements, GC-rich elements, GU-rich elements and AREs, and has been implicated in the regulation of mRNA alternative splicing as well as the control of mRNA translation and stability. CUGBP1 is ubiquitously expressed with localization in nucleus and cytoplasm as a component of an EIF2 complex which contains CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. CUGBP1 also associates with polysomes and interacts with HNRNPH1 and PARN. CUGBP1 was originally discovered as a protein which interacts with RNA CUG repeats that are expanded in patients with DM1 (myotonic dystrophy), which binds to 3'-untranslated region of the dystrophin myotonia protein kinase (DMPK) gene. However, CUGBP1 was later shown as multifunctional protein regulating many posttranscriptional processes including alternative splicing, deadenylation, mRNA decay, and translation. It has been involved in the processes of development (embryonic and cardiac), differentiation (skeletal muscle and adipose tissue), germ cell formation, tumorigenesis (breast cancer, leukemia etc) and deterioration of certain tumors as well.

Long Name

CUGBP, Elav-Like Family Member 1

Alternate Names

BRUNOL2, CELF1, CUG-BP, CUG-BP1, D2Wsu101e, EDEN-BP, HNAB50, NAB50, NAPOR

Entrez Gene IDs

10658 (Human); 13046 (Mouse); 362160 (Rat)

Gene Symbol

CELF1

UniProt

Q92879

Additional CUGBP1/CELF1 Products

Product Documents for CUGBP1/CELF1 Antibody (3B1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CUGBP1/CELF1 Antibody (3B1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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