CUGBP1/CELF1 Antibody (3B1) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80684

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Primate, Rabbit

Applications

Flow Cytometry, Gel Super Shift Assays, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3B1

Format

Azide and BSA Free

Concentration

1 mg/ml

View all CUGBP1/CELF1 Antibodies »

Product Summary for CUGBP1/CELF1 Antibody (3B1) - Azide and BSA Free

Immunogen

Full-length human CUGBP1 [UniProt# Q92879]

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CUGBP1/CELF1 Antibody (3B1) - Azide and BSA Free

Western Blot: CUGBP1/CELF1 Antibody (3B1)Azide and BSA Free [NBP2-80684]

Western Blot: CUGBP1/CELF1 Antibody (3B1)Azide and BSA Free [NBP2-80684]

Western Blot: CUGBP1/CELF1 Antibody (3B1) - Azide and BSA Free [NBP2-80684] - Detection of CUG-BP1 in several cell lysates. Image from the standard format of this antibody.
Immunocytochemistry: CUGBP1/CELF1 Antibody (3B1) - Azide and BSA Free [NBP2-80684]

Immunocytochemistry: CUGBP1/CELF1 Antibody (3B1) - Azide and BSA Free [NBP2-80684]

Immunocytochemistry: CUGBP1/CELF1 Antibody (3B1) - Azide and BSA Free [NBP2-80684] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human spleen using 1:100 conc. of CUGBP1/CELF1 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 30 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate
Flow Cytometry: CUGBP1/CELF1 Antibody (3B1) - Azide and BSA Free [NBP2-80684]

Flow Cytometry: CUGBP1/CELF1 Antibody (3B1) - Azide and BSA Free [NBP2-80684]

Flow Cytometry: CUGBP1/CELF1 Antibody (3B1) - Azide and BSA Free [NBP2-80684] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using CUGBP1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used. Image from the standard format of this antibody.
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Applications for CUGBP1/CELF1 Antibody (3B1) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1 ug per million cells

Gel Super Shift Assays

reported in scientific literature

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Frozen

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

reported in scientific literature

Simple Western

1:200

Western Blot

1:500
Application Notes
In Western Blot, a band is observed at approx. 50 kDa. Nuclear staining can be seen in ICC/IF.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Tris-Glycine, 0.15 M NaCl

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CUGBP1/CELF1

CUGBP1 (CUG-binding protein 1) is a member of CELF (CUG-BP- and ETR-3-like factors) family of RNA-binding proteins that binds to a variety of mRNA cis-elements including UG-rich elements, GC-rich elements, GU-rich elements and AREs, and has been implicated in the regulation of mRNA alternative splicing as well as the control of mRNA translation and stability. CUGBP1 is ubiquitously expressed with localization in nucleus and cytoplasm as a component of an EIF2 complex which contains CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. CUGBP1 also associates with polysomes and interacts with HNRNPH1 and PARN. CUGBP1 was originally discovered as a protein which interacts with RNA CUG repeats that are expanded in patients with DM1 (myotonic dystrophy), which binds to 3'-untranslated region of the dystrophin myotonia protein kinase (DMPK) gene. However, CUGBP1 was later shown as multifunctional protein regulating many posttranscriptional processes including alternative splicing, deadenylation, mRNA decay, and translation. It has been involved in the processes of development (embryonic and cardiac), differentiation (skeletal muscle and adipose tissue), germ cell formation, tumorigenesis (breast cancer, leukemia etc) and deterioration of certain tumors as well.

Long Name

CUGBP, Elav-Like Family Member 1

Alternate Names

BRUNOL2, CELF1, CUG-BP, CUG-BP1, D2Wsu101e, EDEN-BP, HNAB50, NAB50, NAPOR

Gene Symbol

CELF1

Additional CUGBP1/CELF1 Products

Product Documents for CUGBP1/CELF1 Antibody (3B1) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CUGBP1/CELF1 Antibody (3B1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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