Cullin 2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49873

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all CUL2 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QYIERSQASADE corresponding to C-Terminus according to NP_003582.2.

Epitope

QYIERSQASADE - C Terminus

Reactivity Notes

Expected from sequence similarity: Rat, Canine, Bovine

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Cullin 2 Antibody

Western Blot: Cullin 2 Antibody [NBP1-49873]

Western Blot: Cullin 2 Antibody [NBP1-49873]

Western Blot: Cullin 2 Antibody [NBP1-49873] - Analysis of Cullin 2 in NIH3T3 lysate (35ug protein in RIPA buffer) using NBP1-49873 at 1 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Cullin 2 Antibody [NBP1-49873]

Immunohistochemistry-Paraffin: Cullin 2 Antibody [NBP1-49873]

Immunohistochemistry-Paraffin: Cullin 2 Antibody [NBP1-49873] - 3.75ug/ml staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Cullin 2 Antibody

Application
Recommended Usage

Immunohistochemistry

1-3ug/ml

Peptide ELISA

Detection limit 1:8000

Western Blot

1:1000
Application Notes
Western blot: Approx 100kDa band observed in lysates of cell line NIH3T3 (calculated MW of 87.0kDa according to Human NP_003582.2 and of 86.9kDa according to Mouse NP_083678.2). Primary incubation was 1 hour
IHC: Paraffin embedded Human Breast.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CUL2

Cullin proteins comprise a distinct family of mediators that participate in the selective targeting of proteins for ubiquitin (Ub)-mediated proteolysis. CUL-1, which is the mammalian homolog of Cdc53 from yeast, is an integral component of the E3 ubiquitin ligase complex designated SCF. The SCF (Skp1/CUL-1/F-box protein complex) consists of Skp1 associating with both CUL-1 and an F-box protein, such as Skp2, which determines the substrate specificity of the complex. CUL-1 mediated ubiquitination results in the degradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cullin, CUL-3 facilitates the degradation of cyclin E independent of SCF activity, while CUL-2 associates with the tumor suppressing protein VHL and elongin B to form VBC complexes, which structurally resemble the SCF ligase. Proteolysis also occurs by way of CUL-4 associating with Nedd-8, a ubiquitin- like protein, where it too functions as an active component of a multifunctional E3 complex. CUL-5, or vasopressin-activated, calcium-mobilizing protein (VACM-1), is also included in the cullin family as it shares substantial sequence homology with CUL-1.

Long Name

Cullin 2

Alternate Names

Cullin 2

Entrez Gene IDs

8453 (Human); 71745 (Mouse); 361258 (Rat)

Gene Symbol

CUL2

UniProt

NP_003582.2 (Human)

Additional CUL2 Products

Product Documents for Cullin 2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Cullin 2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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