CWC15 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-31977

Novus Biologicals, part of Bio-Techne
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NBP2-31977
NBP2-31977-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CWC15 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: RGGRGKGEGDLSQLSKQYSSRDLPSHTKIKYRQTTQDAPEEVRNRDFRRELEERERAAAREKNRDRPTREHTTSSSVS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CWC15 Antibody

Western Blot: CWC15 Antibody [NBP2-31977]

Western Blot: CWC15 Antibody [NBP2-31977]

Western Blot: CWC15 Antibody [NBP2-31977] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunocytochemistry/ Immunofluorescence: CWC15 Antibody [NBP2-31977]

Immunocytochemistry/ Immunofluorescence: CWC15 Antibody [NBP2-31977]

Immunocytochemistry/Immunofluorescence: CWC15 Antibody [NBP2-31977] - Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & mitochondria.
Immunohistochemistry-Paraffin: CWC15 Antibody [NBP2-31977]

Immunohistochemistry-Paraffin: CWC15 Antibody [NBP2-31977]

Immunohistochemistry-Paraffin: CWC15 Antibody [NBP2-31977] - Staining of human thyroid gland shows strong nuclear positivity in glandular cells.

Applications for CWC15 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CWC15

CWC15, or Spliceosome-associated protein CWC15 homolog, consists of a 229 amino acid isoform that is 27 kDa, and is involved in stimulating pre-mRNA splicing during transcription. Current research is being conducted on CWC15 and its relation to a variety of diseases and disorders, including inclusion conjunctivitis, ADHD, conduct disorder, and laryngotracheitis. CWC15 has been linked to the mRNA splicing pathway, and it interacts with proteins such as CTNNBL1, CCT5, CDC5L, RBM17, and ARHGAP17.

Alternate Names

C11orf5ORF5, CWC15 homolog, CWC15 homolog (S. cerevisiae), CWC15 spliceosome-associated protein homolog (S. cerevisiae), Cwf15, HSPC148, protein CWC15 homolog

Gene Symbol

CWC15

UniProt

None (Human)

Additional CWC15 Products

Product Documents for CWC15 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CWC15 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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