CXCL12/SDF-1 alpha Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29480
![Immunohistochemistry-Paraffin: CXCL12/SDF-1 alpha Antibody - Azide and BSA Free [NBP2-29480]](https://resources.bio-techne.com/images/products/CXCL12-SDF-1-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-29480-img0007.jpg "Immunohistochemistry-Paraffin: CXCL12/SDF-1 alpha Antibody - Azide and BSA Free [NBP2-29480]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Rat
Applications
Validated:
ELISA, Functional, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Recombinant human SDF-1alpha
Specificity
NBP2-29480 recognises human SDF 1 alpha, otherwise known as CXCL12a, a 68 amino acid stromal cell derived CXC chemokine, which arises from alternative splicing of the SDF 1 gene. The two isoforms, SDF 1 alpha and SDF 1 beta, share an identical amino acid sequence, except for an additional four residues in the C terminal region of the beta isoform.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CXCL12/SDF-1 alpha Antibody
Immunohistochemistry-Paraffin: CXCL12/SDF-1 alpha Antibody - Azide and BSA Free [NBP2-29480]
Immunohistochemistry-Paraffin: CXCL12/SDF-1 alpha Antibody [NBP2-29480] - Formalin fixed paraffin embedded tissue section of human colorectal carcinoma stained using SDF-1 alpha antibody (NBP2-29480) with DAB detection. Sodium citrate buffer (pH6.0) based heat mediated antigen retrieval was employed in this IHC-P validation.![Immunohistochemistry-Paraffin: CXCL12/SDF-1 alpha Antibody - Azide and BSA Free [NBP2-29480]](https://resources.bio-techne.com/images/products/CXCL12-SDF-1-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-29480-img0006.jpg "Immunohistochemistry-Paraffin: CXCL12/SDF-1 alpha Antibody - Azide and BSA Free [NBP2-29480]")
Immunohistochemistry-Paraffin: CXCL12/SDF-1 alpha Antibody - Azide and BSA Free [NBP2-29480]
Immunohistochemistry-Paraffin: CXCL12/SDF-1 alpha Antibody [NBP2-29480] - Formalin fixed paraffin embedded tissue section of human colorectal carcinoma stained using SDF-1 alpha antibody (NBP2-29480) with DAB detection. Sodium citrate buffer (pH6.0) based heat mediated antigen retrieval was employed in this IHC-P validation.Applications for CXCL12/SDF-1 alpha Antibody
Application
Recommended Usage
ELISA
0.5-2.0ug/ml
Functional
2.0-4.0ug/ml
Immunohistochemistry-Paraffin
0.5ug/ml
Western Blot
0.1-0.2ug/ml
Application Notes
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose. NBP2-29480 may be used under either reducing or non reducing western blotting conditions. Use in Immunohistochemistry reported in scientific literature (PMID: 31116013).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
Reconstitute with 0.1 ml distilled water. Add 0.09% Sodium Azide for long term storage.
Formulation
PBS
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CXCL12/SDF-1 alpha
Additional CXCL12/SDF-1 alpha Products
Product Documents for CXCL12/SDF-1 alpha Antibody
Product Specific Notices for CXCL12/SDF-1 alpha Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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