CXCL12/SDF-1 beta Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05873

Novus Biologicals, part of Bio-Techne
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NBP3-05873-100ul
NBP3-05873-50ul
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Key Product Details

Species Reactivity

Validated:

Mouse

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CXCL12/SDF-1 beta Antibodies »

Product Summary for CXCL12/SDF-1 beta Antibody

Immunogen

Produced in rabbits immunized with purified, recombinant Mouse CXCL12/SDF-1 beta (Uniprot#: P40224-1; Lys22-Lys89)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for CXCL12/SDF-1 beta Antibody

Western Blot: CXCL12/SDF-1 beta Antibody [NBP3-05873]

Western Blot: CXCL12/SDF-1 beta Antibody [NBP3-05873]

Western Blot: CXCL12/SDF-1 beta Antibody [NBP3-05873] - Lane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:10 kDaObserved band size:13 kDa

Applications for CXCL12/SDF-1 beta Antibody

Application
Recommended Usage

ELISA

1:25000-1:50000

Western Blot

1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CXCL12/SDF-1 beta

CXCL12/Stromal Cell-derived Factor 1 (SDF-1) is a member of the CXC chemokine ligand superfamily. Six alternatively spliced CXCL12/SDF-1 isoforms have been identified including, CXCL12/SDF-1 alpha, CXCL12/SDF-1 beta, and CXCL12/SDF-1 gamma. CXCL12/SDF-1 alpha is 89 amino acids (aa) in length with a predicted molecular weight of 10 kDa, CXCL12/SDF-1 beta is 93 aa in length with a predicted molecular weight of 10.6 kDa, and CXCL12/SDF-1 gamma is 119 aa in length with a predicted molecular weight of 13.7 kDa. The mouse CXCL12/SDF-1 orthologs share 99% aa sequence identity with the human protein. The alpha and beta isoforms are ubiquitously expressed while the gamma isoform is primarily expressed in the heart. CXCL12/SDF-1 isoforms are chemotactic for T lymphocytes and monocytes. It is cardio protective and has also been shown to hinder CXCR4-mediated HIV infection of T cells.

Alternate Names

SDF1 beta, SDF1b

Gene Symbol

CXCL12

Additional CXCL12/SDF-1 beta Products

Product Documents for CXCL12/SDF-1 beta Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CXCL12/SDF-1 beta Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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