CXCR3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-77442

Novus Biologicals, part of Bio-Techne
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NBP2-77442
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CXCR3 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-RRDSSWSETSEA, from the C Terminus according to NP_001495.1; NP_001136269.1.

Epitope

RRDSSWSETSEA

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for CXCR3 Antibody

Immunohistochemistry-Paraffin: CXCR3 Antibody [NBP2-77442]

Immunohistochemistry-Paraffin: CXCR3 Antibody [NBP2-77442]

Immunohistochemistry-Paraffin: CXCR3 Antibody [NBP2-77442] - Staining (8ug/ml) of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-Staining.
Flow Cytometry: CXCR3 Antibody [NBP2-77442]

Flow Cytometry: CXCR3 Antibody [NBP2-77442]

Flow Cytometry: CXCR3 Antibody [NBP2-77442] - Analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunohistochemistry-Paraffin: CXCR3 Antibody [NBP2-77442]

Immunohistochemistry-Paraffin: CXCR3 Antibody [NBP2-77442]

Immunohistochemistry-Paraffin: CXCR3 Antibody [NBP2-77442] - Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.
View 2 more images

Applications for CXCR3 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunohistochemistry-Paraffin

6 - 8 ug/ml

Peptide ELISA

Detection limit 1:64000
Application Notes
WB: Preliminary experiments showed a band at approx 55-60k Da in Human Tonsil and Mouse Spleen lysates after 1ug/ml antibody staining. Please note we cannot currently find an explanation in the literature for this band, given the calculated size of 45.5kDa according to NP_001136269.1

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CXCR3

CD183 is a G protein-coupled receptor with selectivity for three chemokines, termed IP10 (interferon-g-inducible 10 kDa protein), Mig (monokine induced by interferon-g) and I-TAC (interferon-inducible T cell a-chemoattractant). IP10, Mig and I-TAC belong to the structural subfamily of CXC chemokines, in which a single amino acid residue separates the first two of four highly conserved Cys residues. Historically, CD183 is the third CXC chemokine receptor discovered and, therefore, commonly designated as CXCR3. Binding of chemokines to CD183 induces cellular responses that are involved in leukocyte traffic, most notably integrin activation, cytoskeletal changes and chemotactic migration. Inhibition by Bordetella pertussis toxin suggests that heterotrimeric G protein of the Gi-subclass couple to CD183. Signal transduction has not been further analyzed but may include the same enzymes that were identified in the signaling cascade induced by other chemokine receptors. As a consequence of chemokine-induced cellular desensitization (phosphorylation-dependent receptor internalization), cellular responses are typically rapid and short in duration. Cellular responsiveness is restored after dephosphorylation of intracellular receptors and subsequent recycling to the cell surface. A hallmark of CD183 is its prominent expression in in vitro cultured effector/memory T cells, and in T cells present in many types of inflamed tissues. In addition, IP10, Mig and I-TAC are commonly produced by local cells in inflammatory lesion, suggesting that CD183 and its chemokines participate in the recruitment of inflammatory cells. Therefore, CD183 is a target for the development of small molecular weight antagonists, which may be used in the treatment of diverse inflammatory diseases.

Alternate Names

CD183, CXCR3, GPR9

Gene Symbol

CXCR3

Additional CXCR3 Products

Product Documents for CXCR3 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CXCR3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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