CXCR5 Antibody (9Q10I2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16723

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16723-100ul
NBP3-16723-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9Q10I2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CXCR5 Antibodies »

Product Summary for CXCR5 Antibody (9Q10I2)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 273-372 of human CXCR5 (P32302). SPYHIVIFLDTLARLKAVDNTCKLNGSLPVAITMCEFLGLAHCCLNPMLYTFAGVKFRSDLSRLLTKLGCTGPASLCQLFPSWRRSSLSESENATSLTTF

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CXCR5 Antibody (9Q10I2)

Western Blot: CXCR5 Antibody (9Q10I2) [NBP3-16723]

Western Blot: CXCR5 Antibody (9Q10I2) [NBP3-16723]

Western Blot: CXCR5 Antibody (9Q10I2) [NBP3-16723] - Western blot analysis of extracts of various cell lines, using CXCR5 Rabbit mAb (NBP3-16723) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
CXCR5 Antibody (9Q10I2)

Immunocytochemistry/Immunofluorescence:CXCR5 Antibody (9Q10I2) [NBP3-16723] -

Immunocytochemistry/Immunofluorescence:CXCR5 Antibody (9Q10I2) [NBP3-16723] - Confocal imaging of human tonsil using CXCR5 Rabbit mAb (dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 40x.Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.

Applications for CXCR5 Antibody (9Q10I2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CXCR5

Chemokines play important roles in inflammation and critical for the recruitment of effector immune cells to sites of infection. Chemokines activate leukocytes by binding to G protein coupled receptors (1). The ever-growing chemokine receptor subtypes can be divided into 2 major groups, CXCR and CCR, based on the 2 major classes of chemokines. One of the CCR receptors, CCR1, is expressed on neutrophils, monocytes, lymphocytes, and eosinophils and binds the leukocyte chemoattractant and hemopoiesis regulator macrophage-inflammatory protein (MIP-1 ), eotaxin, as well as several other related chemokines (2-4). Mice lacking the chemokine receptor CCR1 have defects in neutrophil trafficking and proliferation (5,6).

Alternate Names

BLR1, CD185, CXCR5

Gene Symbol

CXCR5

Additional CXCR5 Products

Product Documents for CXCR5 Antibody (9Q10I2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CXCR5 Antibody (9Q10I2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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