CYB5R3 Antibody (OTI2A10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02705

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Canine, Monkey, Primate

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI2A10

Concentration

0.76 mg/ml

View all CYB5R3 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human CYB5R3 (NP_000389) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

34.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CYB5R3 Antibody (OTI2A10)

Western Blot: CYB5R3 Antibody (2A10) [NBP2-02705] Analysis of extracts (35ug) from 9 different cell lines by using anti-CYB5R3 monoclonal antibody.
Immunohistochemistry-Paraffin: CYB5R3 Antibody (OTI2A10) [NBP2-02705]

Immunohistochemistry-Paraffin: CYB5R3 Antibody (OTI2A10) [NBP2-02705]

Immunohistochemistry-Paraffin: CYB5R3 Antibody (2A10) [NBP2-02705] - Staining of paraffin-embedded Human liver tissue using anti-CYB5R3 mouse monoclonal antibody.
Flow Cytometry: CYB5R3 Antibody (OTI2A10) [NBP2-02705]

Flow Cytometry: CYB5R3 Antibody (OTI2A10) [NBP2-02705]

Flow Cytometry: CYB5R3 Antibody (2A10) [NBP2-02705] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CYB5R3 antibody, and then analyzed by flow cytometry.
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Applications for CYB5R3 Antibody (OTI2A10)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:200-500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.76 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CYB5R3

This gene encodes cytochrome b5 reductase, which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum, mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids, in cholesterol biosynthesis, and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains, while the soluble form has only the catalytic domain. Alternate splicing results in multiple transcript variants. Mutations in this gene cause methemoglobinemias. [provided by RefSeq].

Alternate Names

B5RNADH-cytochrome b5 reductase 3, Cytochrome b5 reductase, cytochrome b5 reductase 3, DIA1NADH-cytochrome b5 reductase 3 membrane-bound form, diaphorase (NADH) (cytochrome b-5 reductase), diaphorase-1, EC 1.6.2.2, NADH-cytochrome b5 reductase 3 soluble form

Entrez Gene IDs

1727 (Human)

Gene Symbol

CYB5R3

Additional CYB5R3 Products

Product Documents for CYB5R3 Antibody (OTI2A10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CYB5R3 Antibody (OTI2A10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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