CYB5R3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # H00001727-B01P

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Mouse IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CYB5R3 Antibodies »

Product Specifications

Immunogen

CYB5R3 (NP_000389.1, 1 a.a. - 301 a.a.) full-length human protein. MGAQLSTLGHMVLFPVWFLYSLLMKLFQRSTPAITLESPDIKYPLRLIDREIISHDTRRFRFALPSPQHILGLPVGQHIYLSARIDGNLVVRPYTPISSDDDKGFVDLVIKVYFKDTHPKFPAGGKMSQYLESMQIGDTIEFRGPSGLLVYQGKGKFAIRPDKKSNPIIRTVKSVGMIAGGTGITPMLQVIRAIMKDPDDHTVCHLLFANQTEKDILLRPELEELRNKHSARFKLWYTLDRAPEAWDYGQGFVNEEMIRDHLPPPEEEPLVLMCGPPPMIQYACLPNLDHVGHPTERCFVF

Specificity

CYB5R3 - cytochrome b5 reductase 3,

Clonality

Polyclonal

Host

Mouse

Isotype

IgG

Description

Quality control test: Antibody reactive against mammalian transfected lysate.

Scientific Data Images for CYB5R3 Antibody

Western Blot: CYB5R3 Antibody [H00001727-B01P]

Western Blot: CYB5R3 Antibody [H00001727-B01P]

Western Blot: CYB5R3 Antibody [H00001727-B01P] - Analysis of CYB5R3 expression in transfected 293T cell line by CYB5R3 polyclonal antibody. Lane 1: CYB5R3 transfected lysate(33.11 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: CYB5R3 Antibody [H00001727-B01P]

Immunocytochemistry/ Immunofluorescence: CYB5R3 Antibody [H00001727-B01P]

Immunocytochemistry/Immunofluorescence: CYB5R3 Antibody [H00001727-B01P] - Analysis of purified antibody to CYB5R3 on HeLa cell. (antibody concentration 10 ug/ml)

Applications for CYB5R3 Antibody

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control. It has been used for IF.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CYB5R3

This gene encodes cytochrome b5 reductase, which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum, mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids, in cholesterol biosynthesis, and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains, while the soluble form has only the catalytic domain. These two forms are resulted from alternative splicing of the gene. Mutations in this gene cause methemoglobinemias. [provided by RefSeq]

Alternate Names

B5RNADH-cytochrome b5 reductase 3, Cytochrome b5 reductase, cytochrome b5 reductase 3, DIA1NADH-cytochrome b5 reductase 3 membrane-bound form, diaphorase (NADH) (cytochrome b-5 reductase), diaphorase-1, EC 1.6.2.2, NADH-cytochrome b5 reductase 3 soluble form

Entrez Gene IDs

1727 (Human)

Gene Symbol

CYB5R3

UniProt

NP_000389.1 (Human)

Additional CYB5R3 Products

Product Documents for CYB5R3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CYB5R3 Antibody

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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