CYBB/NOX2 Antibody (NL7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-41012

Novus Biologicals, part of Bio-Techne
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NBP1-41012-0.025mg
NBP1-41012-0.1mg
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Flow (Intracellular), Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # NL7

Concentration

1.0 mg/ml

View all CYBB/NOX2 Antibodies »

Product Specifications

Immunogen

Cytochrome B245 solubilized in octyl-beta-glucopyranoside

Epitope

Clone NL7 binds amino acids 498-506 (EKDVITGLK), a cytosolic epitope on gp91phox not accessible on intact neutrophils.

Specificity

This antibody recognises the heavy chain of cytochrome B245. It recognises the native as well as the denatured form of the protein

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CYBB/NOX2 Antibody (NL7)

Flow Cytometry: CYBB/NOX2 Antibody (NL7) - BSA Free [NBP1-41012]

Flow Cytometry: CYBB/NOX2 Antibody (NL7) - BSA Free [NBP1-41012]

Flow Cytometry: CYBB/NOX2 Antibody (NL7) [NBP1-41012] - Using the Alexa Fluor 647 direct conjugate An intracellular stain was performed on Daudi cells with CYBB/NOX2 (NL7) antibody NBP1-41012AF647 (blue) and a matched isotype control NBP1-97005AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
Flow Cytometry: CYBB/NOX2 Antibody (NL7) - BSA Free [NBP1-41012]

Flow Cytometry: CYBB/NOX2 Antibody (NL7) - BSA Free [NBP1-41012]

Flow Cytometry: CYBB/NOX2 Antibody (NL7) [NBP1-41012] - Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Heavy Chain. Permeabilised with Leucoperm
Flow Cytometry: CYBB/NOX2 Antibody (NL7) - BSA Free [NBP1-41012]

Flow Cytometry: CYBB/NOX2 Antibody (NL7) - BSA Free [NBP1-41012]

Flow Cytometry: CYBB/NOX2 Antibody (NL7) [NBP1-41012] - Analysis using the PE conjugate of NBP1-41012. Staining of CYBB in human peripheral blood using anti-CYBB antibody. Image from verified customer review.
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Applications for CYBB/NOX2 Antibody (NL7)

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
For Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul (Burrit, J. B. et al.). Western blot detects a broad band at ~90 kDa. Clone NL7 has an inhibitory effect on NADPH oxidase activation. The antibody does not interfere with the translocation of the cytosolic subunits of NADPH oxidase to the cell membrane.

Reviewed Applications

Read 2 reviews rated 4.5 using NBP1-41012 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CYBB/NOX2

Cytochrome B245, also known as gp91phox, a subunit of the nicotinamide adenine dinucleotide phosphate-oxidase (NADPH oxidase). NADPH oxidase is a membrane-bound enzyme complex in neutrophils that produces the superoxide anion (O2-) by transferring single electrons from NADPH inside the cell across the membrane and coupling them to molecular oxygen. Superoxide is used for the destruction of pathogens. Individuals with defects in NADPH oxidase suffer from chronic granulomatous disease, characterised by severe recurrent infections. The heavy chain (gp91phox), along with the light chain (p22phox) form cytochrome B245 (also known as flavocytochome b, or Cyt b), which is always membrane-resident. Upon the appropriate stimulus, other components of NADPH oxidase (p47phox, p40phox, p67phox and Rac2) rapidly translocate from the cytosol to the cell membrane to form the complete enzyme complex. Cytochrome B245 appears to serve as a scaffold for the assembly of the other cytosolic units of the oxidase and to provide a transmembrane pathway for electrons.

Long Name

Cytochrome b-245 heavy chain

Alternate Names

CGD91-phox, CYBB, EC 1.-.-.-, gp91-1, gp91-phox, NADPH oxidase 2, NOX2

Entrez Gene IDs

1536 (Human)

Gene Symbol

CYBB

UniProt

P04839 (Human)

Additional CYBB/NOX2 Products

Product Documents for CYBB/NOX2 Antibody (NL7)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CYBB/NOX2 Antibody (NL7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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