Cyclin A2 Antibody (3E9Z3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15625

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15625-100ul
NBP3-15625-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3E9Z3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Cyclin A2 Antibodies »

Product Summary for Cyclin A2 Antibody (3E9Z3)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 11-210 of human Cyclin A2 (P20248). TREAGSALLALQQTALQEDQENINPEKAAPVQQPRTRAALAVLKSGNPRGLAQQQRPKTRRVAPLKDLPVNDEHVTVPPWKANSKQPAFTIHVDEAEKEAQKKPAESQKIEREDALAFNSAISLPGPRKPLVPLDYPMDGSFESPHTMDMSIILEDEKPVSVNEVPDYHEDIHTYLREMEVKCKPKVGYMKKQPDITNSM

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cyclin A2 Antibody (3E9Z3)

Western Blot: Cyclin A2 Antibody (3E9Z3) [NBP3-15625]

Western Blot: Cyclin A2 Antibody (3E9Z3) [NBP3-15625]

Western Blot: Cyclin A2 Antibody (3E9Z3) [NBP3-15625] - Western blot analysis of extracts of various cell lines, using Cyclin A2 antibody (NBP3-15625) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: Cyclin A2 Antibody (3E9Z3) [NBP3-15625]

Immunocytochemistry/ Immunofluorescence: Cyclin A2 Antibody (3E9Z3) [NBP3-15625]

Immunocytochemistry/Immunofluorescence: Cyclin A2 Antibody (3E9Z3) [NBP3-15625] - Immunofluorescence analysis of U-2 OS cells using Cyclin A2 Rabbit mAb (NBP3-15625) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: Cyclin A2 Antibody (3E9Z3) [NBP3-15625]

Immunohistochemistry-Paraffin: Cyclin A2 Antibody (3E9Z3) [NBP3-15625]

Immunohistochemistry-Paraffin: Cyclin A2 Antibody (3E9Z3) [NBP3-15625] - Immunohistochemistry of paraffin-embedded mouse liver using Cyclin A2 Rabbit mAb (NBP3-15625) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for Cyclin A2 Antibody (3E9Z3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Immunoprecipitation

1:100 - 1:500

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cyclin A2

Alternate Names

CCNA, CCNA2

Gene Symbol

CCNA2

Additional Cyclin A2 Products

Product Documents for Cyclin A2 Antibody (3E9Z3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cyclin A2 Antibody (3E9Z3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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