Cyclin A2 Antibody (E67) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34613

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Bovine, Mink, Monkey

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # E67

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all Cyclin A2 Antibodies »

Product Specifications

Immunogen

Full length bovine Cyclin A2 protein (Uniprot: P20248)

Epitope

aa 144-148

Reactivity Notes

African Green Monkey, Mink (Mustela)

Localization

Predominantly nuclear

Specificity

It recognizes a protein of 54kDa, which is identified as cyclin A. Its epitope is located amino acids 144-148 of human Cyclin A2. Cyclins are regulatory subunits of the cyclin-dependent kinases (cdks. These cyclin/cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, cyclin A is required for S-phase and passage through G2-phase. The D and E type cyclins regulate the passage of G1, while cyclin B is a critical regulator of mitosis. Mutation or disruption of normal cyclin A expression causes cells to arrest in G2-phase.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-34311).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Cyclin A2 Antibody (E67) - Azide and BSA Free

Western Blot: Cyclin A2 Antibody (E67)Azide and BSA Free [NBP2-34613]

Western Blot: Cyclin A2 Antibody (E67)Azide and BSA Free [NBP2-34613]

Western Blot: Cyclin A2 Antibody (E67) - Azide and BSA Free [NBP2-34613] - Western Blot Analysis of HeLa cell lysate using Cyclin A2 Antibody (E67).
Immunohistochemistry-Paraffin: Cyclin A2 Antibody (E67) - Azide and BSA Free [NBP2-34613]

Immunohistochemistry-Paraffin: Cyclin A2 Antibody (E67) - Azide and BSA Free [NBP2-34613]

Immunohistochemistry-Paraffin: Cyclin A2 Antibody (E67) - Azide and BSA Free [NBP2-34613] - Formalin-fixed, paraffin-embedded human Endometrium stained with Cyclin A2 Antibody (E67).

Applications for Cyclin A2 Antibody (E67) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5-1ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2ug/ml

Immunohistochemistry-Paraffin

1-2ug/ml

Western Blot

0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Cyclin A2

Alternate Names

CCNA, CCNA2

Gene Symbol

CCNA2

Additional Cyclin A2 Products

Product Documents for Cyclin A2 Antibody (E67) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cyclin A2 Antibody (E67) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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