Cyclin B1 Antibody (SPM619) [mFluor Violet 500 SE]
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11598MFV500
![Cyclin B1 Antibody (SPM619) [mFluor Violet 500 SE]](https://resources.bio-techne.com/images/products/nbp3-11598mfv500_mouse-cyclin-b1-mab-spm619-mfluor-violet-500-se-57202314435467.png "Cyclin B1 Antibody (SPM619) [mFluor Violet 500 SE] -")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
mFluor Violet 500 SE (Excitation = 410 nm, Emission = 501 nm)
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM619
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Cyclin B1 Antibody (SPM619) [mFluor Violet 500 SE]
Immunogen
Recombinant human full-length Cyclin B1 protein (Uniprot: P14635)
Localization
Cytoplasmic & Nuclear
Specificity
It recognizes a protein of 55-62kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for Cyclin B1 Antibody (SPM619) [mFluor Violet 500 SE]
Cyclin B1 Antibody (SPM619) [mFluor Violet 500 SE] -
Cyclin B1 Antibody (SPM619) [mFluor Violet 500 SE] - Vial of mFluor Violet 500 conjugated antibody. mFluor Violet 500 is optimally excited at 410 nm by the Violet laser (405 nm) and has an emission maximum of 501 nm.Applications for Cyclin B1 Antibody (SPM619) [mFluor Violet 500 SE]
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Cyclin B1
Alternate Names
CCNB1
Gene Symbol
CCNB1
Additional Cyclin B1 Products
Product Documents for Cyclin B1 Antibody (SPM619) [mFluor Violet 500 SE]
Product Specific Notices for Cyclin B1 Antibody (SPM619) [mFluor Violet 500 SE]
mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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