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Cyclin B1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-55200

Novus Biologicals, part of Bio-Techne
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NBP2-55200-25ul
NBP2-55200

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for Cyclin B1 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: PVPVSEPVPEPEPEPEPEPVKEEKLSPEPILVDTASPSPMETSGCAPAEEDLCQAFSDVILAVNDVDAEDGAD

Reactivity Notes

Mouse 80%

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cyclin B1 Antibody

Western Blot: Cyclin B1 Antibody [NBP2-55200]

Western Blot: Cyclin B1 Antibody [NBP2-55200]

Western Blot: Cyclin B1 Antibody [NBP2-55200] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-CCNB1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: Cyclin B1 Antibody [NBP2-55200]

Immunocytochemistry/ Immunofluorescence: Cyclin B1 Antibody [NBP2-55200]

Immunocytochemistry/Immunofluorescence: Cyclin B1 Antibody [NBP2-55200] - Staining of human cell line U-251 MG shows localization to cytosol.

Applications for Cyclin B1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cyclin B1

Alternate Names

CCNB1

Gene Symbol

CCNB1

Additional Cyclin B1 Products

Product Documents for Cyclin B1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Cyclin B1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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