Cyclin D1 Antibody (DCS-6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15189

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Monkey

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Flow Cytometry, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # DCS-6

Concentration

0.2 mg/ml

View all Cyclin D1 Antibodies »

Product Specifications

Immunogen

Human full length recombinant cyclin D1 protein (Uniprot: P24385)

Localization

Nuclear

Specificity

Recognizes a protein of 36kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t(11; 14)(q13; q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative). About 40% of breast carcinomas are positive for Cyclin D1. Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.

Marker

G1-Cyclin & Mantle Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-33138)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Cyclin D1 Antibody (DCS-6)

Western Blot: Cyclin D1 Antibody (DCS-6) [NBP2-15189]

Western Blot: Cyclin D1 Antibody (DCS-6) [NBP2-15189]

Western Blot: Cyclin D1 Antibody (DCS-6) [NBP2-15189] - analysis of Cyclin D1 in 1) C2C12, 2) HepG2, and 3) NIH3T3 lysate probed with Cyclin D1 antibody at 5 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Immunohistochemistry-Paraffin: Cyclin D1 Antibody (DCS-6) [NBP2-15189]

Immunohistochemistry-Paraffin: Cyclin D1 Antibody (DCS-6) [NBP2-15189]

Immunohistochemistry-Paraffin: Cyclin D1 Antibody (DCS-6) [NBP2-15189] - Formalin-fixed, paraffin-embedded human Mantle Cell Lymphoma stained with Cyclin D1 Antibody (DCS-6).
Flow Cytometry: Cyclin D1 Antibody (DCS-6) [NBP2-15189]

Flow Cytometry: Cyclin D1 Antibody (DCS-6) [NBP2-15189]

Flow Cytometry: Cyclin D1 Antibody (DCS-6) [NBP2-15189] - Flow Cytometric analysis of human Cyclin D1 on MCF-7 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled Cyclin D1 Monoclonal Antibody (DCS-6).
View 3 more images

Applications for Cyclin D1 Antibody (DCS-6)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Use in Immunohistochemistry reported in scientific literature (PMID: 30050877)
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cyclin D1

Cyclin D1 is a member of a superfamily of cyclins, proteins that govern transitions through distinct phases of the cell cycle by regulating CDKs. The D-type subfamily includes cyclin D1, D2, and D3. All three are differentially, and sometimes redundantly, expressed in different lineages. However, in every cell type at least one of the three can be detected. Cyclin D1 as well as D2 and D3 have central roles in linking exogenous growth regulating stimuli with the cycling machinery of cells. They are typically considered to be G1 cyclins; synthesis is initiated during G1 and their expression drives the G1/S phase transition.
The deregulation of cyclin D1 or other D types is commonly involved in a wide range of cancers. For example cyclin D1 is activated by chromosomal rearrangements in both parathyroid adenomas and B-cell neoplasms. The DCS-6 antibody is specific for cyclin D1, and has been key in elucidating the function and involvement of cyclin D1 in oncogenesis. Results with this antibody have help to show that Cyclin D1 is over expressed in many different kinds of cancer. For example, about 50-70% of mantle cell lymphomas and 40% of breast carcinomas are cyclin D1.

Alternate Names

Bcl-1, CCND1, Cyl-1, PRAD1

Gene Symbol

CCND1

UniProt

P24385

Additional Cyclin D1 Products

Product Documents for Cyclin D1 Antibody (DCS-6)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Cyclin D1 Antibody (DCS-6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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