Cyclin E1 Antibody (CCNE1/2460)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07484

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # CCNE1/2460

Concentration

0.2 mg/ml

View all Cyclin E1 Antibodies »

Product Summary for Cyclin E1 Antibody (CCNE1/2460)

Immunogen

Recombinant human Cyclin E1 protein fragment (around aa 10-176) (exact sequence is proprietary) (Uniprot: IDP24864)

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08805)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Cyclin E1 Antibody (CCNE1/2460)

Western Blot: Cyclin E1 Antibody (CCNE1/2460) [NBP3-07484]

Western Blot: Cyclin E1 Antibody (CCNE1/2460) [NBP3-07484]

Western Blot: Cyclin E1 Antibody (CCNE1/2460) [NBP3-07484] - Western Blot Analysis of human Jurkat cell lysate using Cyclin E1 Mouse Monoclonal Antibody (CCNE1/2460).
Immunohistochemistry-Paraffin: Cyclin E1 Antibody (CCNE1/2460) [NBP3-07484]

Immunohistochemistry-Paraffin: Cyclin E1 Antibody (CCNE1/2460) [NBP3-07484]

Immunohistochemistry-Paraffin: Cyclin E1 Antibody (CCNE1/2460) [NBP3-07484] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with Cyclin E1 Mouse Monoclonal Antibody (CCNE1/2460).
Immunohistochemistry-Paraffin: Cyclin E1 Antibody (CCNE1/2460) [NBP3-07484]

Immunohistochemistry-Paraffin: Cyclin E1 Antibody (CCNE1/2460) [NBP3-07484]

Immunohistochemistry-Paraffin: Cyclin E1 Antibody (CCNE1/2460) [NBP3-07484] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with Cyclin E1 Mouse Monoclonal Antibody (CCNE1/2460).
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Applications for Cyclin E1 Antibody (CCNE1/2460)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cyclin E1

Alternate Names

CCNE, CCNE1

Gene Symbol

CCNE1

Additional Cyclin E1 Products

Product Documents for Cyclin E1 Antibody (CCNE1/2460)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cyclin E1 Antibody (CCNE1/2460)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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