Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # CCNE1/4935R

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Cyclin E1 Antibodies »

Product Specifications

Immunogen

Recombinant human Cyclin E1 protein fragment (around aa 10-176) (exact sequence is proprietary) (Uniprot: IDP24864)

Localization

Nuclear

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07488).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Cyclin E1 Antibody (CCNE1/4935R) - Azide and BSA Free

Western Blot: Cyclin E1 Antibody (CCNE1/4935R)Azide and BSA Free [NBP3-08810]

Western Blot: Cyclin E1 Antibody (CCNE1/4935R)Azide and BSA Free [NBP3-08810]

Western Blot: Cyclin E1 Antibody (CCNE1/4935R) - Azide and BSA Free [NBP3-08810] - Western Blot Analysis of HCT-116 cell lysate using Cyclin E1 Recombinant Rabbit Monoclonal Antibody (CCNE1/4935R).
Immunohistochemistry-Paraffin: Cyclin E1 Antibody (CCNE1/4935R) - Azide and BSA Free [NBP3-08810]

Immunohistochemistry-Paraffin: Cyclin E1 Antibody (CCNE1/4935R) - Azide and BSA Free [NBP3-08810]

Immunohistochemistry-Paraffin: Cyclin E1 Antibody (CCNE1/4935R) - Azide and BSA Free [NBP3-08810] - Formalin-fixed, paraffin-embedded human endometrium stained with Cyclin E1 Recombinant Rabbit Monoclonal Antibody (CCNE1/4935R).
Immunohistochemistry-Paraffin: Cyclin E1 Antibody (CCNE1/4935R) - Azide and BSA Free [NBP3-08810]

Immunohistochemistry-Paraffin: Cyclin E1 Antibody (CCNE1/4935R) - Azide and BSA Free [NBP3-08810]

Immunohistochemistry-Paraffin: Cyclin E1 Antibody (CCNE1/4935R) - Azide and BSA Free [NBP3-08810] - Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with Cyclin E1 Recombinant Rabbit Monoclonal Antibody (CCNE1/4935R).

Applications for Cyclin E1 Antibody (CCNE1/4935R) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Cyclin E1

Alternate Names

CCNE, CCNE1

Gene Symbol

CCNE1

Additional Cyclin E1 Products

Product Documents for Cyclin E1 Antibody (CCNE1/4935R) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Cyclin E1 Antibody (CCNE1/4935R) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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