Cyclin H Antibody (3N5S3)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16325
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 3N5S3
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 224-323 of human Cyclin H (P51946). AGITMESYLSESLMLKENRTCLSQLLDIMKSMRNLVKKYEPPRSEEVAVLKQKLERCHSAELALNVITKKRKGYEDDDYVSKKSKHEEEEWTDDDLVESL
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Cyclin H Antibody (3N5S3)
Western Blot: Cyclin H Antibody (3N5S3) [NBP3-16325]
Western Blot: Cyclin H Antibody (3N5S3) [NBP3-16325] - Western blot analysis of extracts of various cell lines, using Cyclin H Rabbit mAb (NBP3-16325) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.Immunocytochemistry/ Immunofluorescence: Cyclin H Antibody (3N5S3) [NBP3-16325]
Immunocytochemistry/Immunofluorescence: Cyclin H Antibody (3N5S3) [NBP3-16325] - Immunofluorescence analysis of U-2 OS cells using Cyclin H Rabbit mAb (NBP3-16325) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunohistochemistry-Paraffin: Cyclin H Antibody (3N5S3) [NBP3-16325]
Immunohistochemistry-Paraffin: Cyclin H Antibody (3N5S3) [NBP3-16325] - Immunohistochemistry of paraffin-embedded mouse testis using Cyclin H Rabbit mAb (NBP3-16325) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Applications for Cyclin H Antibody (3N5S3)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Cyclin H
Alternate Names
CAK, cyclin H, cyclin-dependent kinase-activating kinase complex subunit, cyclin-H, MO15-associated protein, p34CAK complex subunit, p37CDK-activating kinase complex subunit
Gene Symbol
CCNH
Additional Cyclin H Products
Product Documents for Cyclin H Antibody (3N5S3)
Product Specific Notices for Cyclin H Antibody (3N5S3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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