Cyclophilin 40 Antibody (1B8) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-60239

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 1B8

Format

BSA Free

Concentration

1 mg/ml

View all Cyclophilin 40 Antibodies »

Product Specifications

Immunogen

Recombinant human Cyclophilin 40 (1-370aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for Cyclophilin 40 Antibody (1B8) - BSA Free

Western Blot: Cyclophilin 40 Antibody (1B8)BSA Free [NBP2-60239]

Western Blot: Cyclophilin 40 Antibody (1B8)BSA Free [NBP2-60239]

Western Blot: Cyclophilin 40 Antibody (1B8) [NBP2-60239] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPID antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: K562 cell lysateLane 2.: Jurkat cell lysate Lane 3.: HeLa cell lysate Lane 4.: HepG2 cell lysateLane 5.: A549 cell lysateLane 6.: MCF7 cell lysateLane 7.: SK-OV-3 cell lysateLane 8.: PC3 cell lysate
Immunocytochemistry/ Immunofluorescence: Cyclophilin 40 Antibody (1B8) - BSA Free [NBP2-60239]

Immunocytochemistry/ Immunofluorescence: Cyclophilin 40 Antibody (1B8) - BSA Free [NBP2-60239]

Immunocytochemistry/Immunofluorescence: Cyclophilin 40 Antibody (1B8) [NBP2-60239] - Staining in Hep3B cells. The secondary antibody (green) was Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Applications for Cyclophilin 40 Antibody (1B8) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cyclophilin 40

Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents: cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins. CyP 40, a 40 kDa protein, shares significant homology with smaller CyPA (CyP 18) and FKBP59. CyP 40 exhibits the characteristic CsA binding and isomerase activity of CyP 18, though these activities appear to be less with CyP 40 than with Cyp 18. Like FKBP59, CyP 40 has been found in progesterone receptor complexes. CyP 40 is expressed at similar levels in many tissues.

Alternate Names

40 kDa peptidyl-prolyl cis-trans isomerase, 40 kDa peptidyl-prolyl cis-trans isomerase D, cyclophilin D, Cyclophilin-40, Cyclophilin-related protein, CYP40, CYP-40cyclophilin 40, CYPD, EC 5.2.1.8, MGC33096, peptidyl-prolyl cis-trans isomerase D, peptidylprolyl isomerase D, peptidylprolyl isomerase D (cyclophilin D), PPIase D, Rotamase D

Gene Symbol

PPID

Additional Cyclophilin 40 Products

Product Documents for Cyclophilin 40 Antibody (1B8) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Cyclophilin 40 Antibody (1B8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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