Skip to main content

Cyclophilin A Antibody (4G3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26372

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
NBP3-26372-100ul
NBP3-26372-50ul

Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4G3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthesized peptide derived from Human Cyclophilin A [UniProt P62937]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cyclophilin A Antibody (4G3)

Cyclophilin A Antibody (4G3)

Western Blot: Cyclophilin A Antibody (4G3) [NBP3-26372] -

Western Blot: Cyclophilin A Antibody (4G3) [NBP3-26372] - Positive Western Blot detected in: Raji whole cell lysate, HepG2 whole cell lysate.
All lanes: Cyclophilin A Antibody at 1: 1500
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 19, 12 kDa
Observed band size: 19 kDa

Applications for Cyclophilin A Antibody (4G3)

Application
Recommended Usage

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Cyclophilin A

Cyclophilin A, also known as Peptidylprolyl Isomerase A, PPIA, CYPA, and CYPH, was originally characterized for its ability to catalyze the transition between cis and trans proline residues critical for proper folding of proteins. Ubiquitously expressed, Cyclophilin A has a predicted molecular weight of 17 kDa and is proinflammatory cytokine that is secreted in response to inflammatory stimuli. Human Cyclophilin A shares 95% amino acid identity with both the mouse and rat orthologs. Cyclophilin A is the main target of the immunosuppressive drug Cyclosporin A. The immunosuppressive activity of cyclosporins has been correlated with their ability to form complexes with cyclophilins that inhibit Calcineurin Phosphatase activity and prevent incorporation of Cyclophilin A into viral particles. Cyclophilin A is known to be incorporated into many viruses, including HIV1 and Hepatitis C, where it may be involved in functions such as viral assembly, replication, and infectivity. In addition, Cyclophilin A has been shown to promote atherosclerosis via binding to Extracellular matrix metalloproteinase (MMP) inducer (EMMPRIN) on CD34+ progenitor cells and stimulating differentiation to foam cells, an essential step during atherosclerotic plaque formation in blood vessels. Cyclophilin A-induced disruption of blood-brain barrier function is thought to contribute to the increased risk of developing Alzheimer’s disease in individuals that possess one or two Apolipoprotein E4 alleles.

Alternate Names

CYPA, CYPH, PPIA, Rotamase

Gene Symbol

PPIA

Additional Cyclophilin A Products

Product Documents for Cyclophilin A Antibody (4G3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Cyclophilin A Antibody (4G3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...