Cyclophilin B Antibody (CL3915) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44067

Novus Biologicals, part of Bio-Techne
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NBP3-44067
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NBP2-59782
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL3915

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Cyclophilin B Antibodies »

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of P23284, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Cyclophilin B Antibody (CL3915) - Azide and BSA Free

Cyclophilin B Antibody (CL3915) - Azide and BSA Free Western Blot: Cyclophilin B Antibody [NBP3-44067]

Western Blot: Cyclophilin B Antibody [NBP3-44067]

Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431, human cell line HepG2, mouse cell line NIH-3T3 and rat cell line NBT-II.
Cyclophilin B Antibody (CL3915) - Azide and BSA Free Western Blot: Cyclophilin B Antibody [NBP3-44067]

Western Blot: Cyclophilin B Antibody [NBP3-44067]

Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-Cyclophilin B antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Applications for Cyclophilin B Antibody (CL3915) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cyclophilin B

Cyclophilin B (SCYLP, CyPB and peptidyl-prolyl cis-trans isomerase B) is a 24 kDa glycoprotein member of the B subfamily of the cyclophilin-type PPIase family of molecules. It is both secreted and retained in the ER. When secreted, Cyclophilin B mediates chemotaxis and T cell adhesion to fibronectin. This is likely due to its prolyl cis/trans isomerase activity. Intracellularly, Cyclophilin B appears to serve as a molecular chaperone for molecules destined for secretion. It does so via stabilization, and facilitating the activity of additional chaperones. The human Cyclophilin B precursor is 216 amino acids (aa) in length. It contains a 25 aa signal sequence plus a 191 aa mature region. There is a partial heparin-binding sequence (aa 27-34), a PPIase domain (aa 47-204) and a C-terminal ER retention motif (aa 213-216). Over aa 34-216, the human and mouse sequences are 95% aa identical.

Alternate Names

CYP-S1, CYPB, PPIB, S-cyclophilin, SCYLP

Gene Symbol

PPIB

Additional Cyclophilin B Products

Product Documents for Cyclophilin B Antibody (CL3915) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Cyclophilin B Antibody (CL3915) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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