Cyclophilin B Antibody (M019)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23271

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # M019

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Cyclophilin B Antibodies »

Product Specifications

Immunogen

Clone M019 was generated from a proprietary antigen related to cyclophilin B from the MCF7 breast cancer cell line. [UniProt# P23284]

Specificity

Clone M019 detects a 20 kDa band corresponding to the molecular mass of cyclophilin B on SDS-PAGE immunoblots of human LNCaP, MeWo, MCF7, and MDA-MB-231 cell lysates, as well as full length recombinant cyclophilin B, but does not detect recombinant cyclophilin A.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for Cyclophilin B Antibody (M019)

Cyclophilin B Antibody (M019)

Western Blot: Cyclophilin B Antibody (M019) [NBP3-23271] -

Western Blot: Cyclophilin B Antibody (M019) [NBP3-23271] - Western blot analysis of human LNCaP (lane 1), MCF7 (lane 2), MDA-MB-231 (lane 3), and MeWo (lane 4) cell lysates, as well as human recombinant full-length cyclophilin B (lane 5) and cyclophilin A (lane 6). The blot was probed with mouse monoclonal anti-cyclophilin B (NBP3-23271) at 1:500.
Cyclophilin B Antibody (M019)

Immunocytochemistry/Immunofluorescence: Cyclophilin B Antibody (M019) [NBP3-23271] -

Immunocytochemistry/Immunofluorescence: Cyclophilin B Antibody (M019) [NBP3-23271] - Immunocytochemical labeling of cyclophilin B in methanol:acetone (1:1) fixed human A549 lung cancer cells. The cells were labeled with mouse monoclonal anti-cyclophilin B (NBP3-23271). The antibody was detected using goat anti-mouse DyLight(R) 594.

Applications for Cyclophilin B Antibody (M019)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunoprecipitation

1:100

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Cyclophilin B

Cyclophilin B (SCYLP, CyPB and peptidyl-prolyl cis-trans isomerase B) is a 24 kDa glycoprotein member of the B subfamily of the cyclophilin-type PPIase family of molecules. It is both secreted and retained in the ER. When secreted, Cyclophilin B mediates chemotaxis and T cell adhesion to fibronectin. This is likely due to its prolyl cis/trans isomerase activity. Intracellularly, Cyclophilin B appears to serve as a molecular chaperone for molecules destined for secretion. It does so via stabilization, and facilitating the activity of additional chaperones. The human Cyclophilin B precursor is 216 amino acids (aa) in length. It contains a 25 aa signal sequence plus a 191 aa mature region. There is a partial heparin-binding sequence (aa 27-34), a PPIase domain (aa 47-204) and a C-terminal ER retention motif (aa 213-216). Over aa 34-216, the human and mouse sequences are 95% aa identical.

Alternate Names

CYP-S1, CYPB, PPIB, S-cyclophilin, SCYLP

Gene Symbol

PPIB

Additional Cyclophilin B Products

Product Documents for Cyclophilin B Antibody (M019)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Cyclophilin B Antibody (M019)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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