Cyr61/CCN1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-356

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rabbit

Cited:

Human, Mouse, Rabbit

Applications

Validated:

Block/Neutralize, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Cited:

Block/Neutralize, Functional Assay, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all Cyr61/CCN1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to the human CYR61 protein sequence (between residues 150-250). [UniProt# O00622]

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 22401280). Mouse reactivity reported in scientific literature (PMID: 27776183).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cyr61/CCN1 Antibody - BSA Free

Immunohistochemistry: Cyr61/CCN1 Antibody - BSA Free [NB100-356]

Immunohistochemistry: Cyr61/CCN1 Antibody - BSA Free [NB100-356]

Immunohistochemistry: Cyr61/CCN1 Antibody [NB100-356] - Sections of the ectopic lesions collected from E2 or E2 plus P4-treated recipients (D16, n = 6) were subjected to histological examination. (B) Representative images (20X) showing IHC analysis using antibodies against an angiogenetic regulator CCN1. The immunostaining intensity of CCN1 was analyzed by ImageJ software. The numerical values were analyzed by One-way ANOVA followed by Dunnett's post hoc test and expressed as mean +/- SEM. Statistical significance is defined as *: p<0.01. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0165347) licensed under a CC-BY license.
Western Blot: Cyr61/CCN1 AntibodyBSA Free [NB100-356]

Western Blot: Cyr61/CCN1 AntibodyBSA Free [NB100-356]

Western Blot: Cyr61/CCN1 Antibody [NB100-356] - Total protein from Human liver, spleen and HepG2 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-CYR61 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the low expression level of CYR61 in HepG2 cells.
Immunocytochemistry/ Immunofluorescence: Cyr61/CCN1 Antibody - BSA Free [NB100-356]

Immunocytochemistry/ Immunofluorescence: Cyr61/CCN1 Antibody - BSA Free [NB100-356]

Immunocytochemistry/Immunofluorescence: Cyr61/CCN1 Antibody [NB100-356] - MCF7 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Cyr61/CCN1 (NB100-356) at 1:200 overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at 1:500. Alpha tubulin was used as a co-stain at 1:1000 and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
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Applications for Cyr61/CCN1 Antibody - BSA Free

Application
Recommended Usage

Block/Neutralize

reported in scientific literature (PMID 22282654)

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 18202125)

Immunohistochemistry

reported in scientific literature

Immunohistochemistry-Paraffin

1:100

Simple Western

1:400

Western Blot

1:200
Application Notes
In Western blot a band is observed at ~37 kDa, representing the processed form of CYR61 protein. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using NB100-356 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cyr61/CCN1

CYR61 (cysteine-rich angiogenic inducer 61), a heparin-binding growth factor, belongs to CCN family whose members are: cysteine-rich 61 (CYR61/CCN1), connective tIssue growth factor (CTGF/CCN2), nephroblastoma overexpressed (NOV/CCN3), and Wnt-induced secreted proteins-1 (WISP-1/CCN4), -2 (WISP-2/CCN5) and -3 (WISP-3/CCN6). CYR61 localizes intracellularly or associates with extracellular matrix at cell surfaces and its reported nuclear localization is surprising, since the primary structure of CYR61 does not include a classic nuclear localization sequence, however, like NOV, EGF, FGF, PDGF, angiogenin, PTHrP etc which lack afore-mentioned sequence, CYR61's nuclear presence may be correlated with transcriptional regulation, and/or mRNA transport. CYR61 plays a key role in wound healing by up-regulating the expression of several genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1, integrins alpha-3 and alpha-5. CYR61 down-regulates the expression of alpha-1/alpha-2 subunits of collagen type-1 and promotes cell adhesion/adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3. CYR61 has been reported to exert its diverse functions via cross-talk with/activating of Wnt, NFkB, AKT and tyrosine kinase signaling pathways.

Long Name

Cysteine-Rich, Angiogenic Inducer 61

Alternate Names

CCN1, GIG1, IGFBP-10

Entrez Gene IDs

3491 (Human)

Gene Symbol

CCN1

UniProt

O00622 (Human)

Additional Cyr61/CCN1 Products

Product Documents for Cyr61/CCN1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Cyr61/CCN1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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