Cystatin E/M/CST6 Antibody (2H8)

Novus Biologicals, part of Bio-Techne | Catalog # H00001474-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2H8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Cystatin E/M Antibodies »

Product Specifications

Immunogen

CST6 (AAH31334, 29 a.a. ~ 149 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RPQERMVGELRDLSPDDPQVQKAAQAAVASYNMGSNSIYYFRDTHIIKAQSQLVAGIKYFLTMEMGSTDCRKTRVTGDHVDLTTCPLAAGAQQEKLRCDFEVLVVPWQNSSQLLKHNCVQM

Specificity

CST6 (2H8)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Cystatin E/M/CST6 Antibody (2H8)

Western Blot: Cystatin E/M/CST6 Antibody (2H8) [H00001474-M01]

Western Blot: Cystatin E/M/CST6 Antibody (2H8) [H00001474-M01]

Western Blot: Cystatin E/M/CST6 Antibody (2H8) [H00001474-M01] - Analysis of CST6 expression in transfected 293T cell line by CST6 monoclonal antibody (M01), clone 2H8.Lane 1: CST6 transfected lysate (Predicted MW: 16.5 KDa).Lane 2: Non-transfected lysate.

Applications for Cystatin E/M/CST6 Antibody (2H8)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Cystatin E/M

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. This gene encodes a cystatin from the type 2 family, which is down-regulated in metastatic breast tumor cells as compared to primary tumor cells. Loss of expression is likely associated with the progression of a primary tumor to a metastatic phenotype.

Alternate Names

CST6

Entrez Gene IDs

1474 (Human)

Gene Symbol

CST6

OMIM

601891 (Human)

UniProt

AAH31334 (Human)

Additional Cystatin E/M Products

Product Documents for Cystatin E/M/CST6 Antibody (2H8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cystatin E/M/CST6 Antibody (2H8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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