Cystatin S Antibody (006)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89913

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP2-89913-50ul
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 006

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Cystatin S Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human Cystatin S (Accession#: NP_001890.1; Met 1-Ala 141).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Cystatin S Antibody (006)

Immunocytochemistry/ Immunofluorescence: Cystatin S Antibody (006) [NBP2-89913]

Immunocytochemistry/ Immunofluorescence: Cystatin S Antibody (006) [NBP2-89913]

Immunocytochemistry/Immunofluorescence: Cystatin S Antibody (006) [NBP2-89913] - Staining of Human CST4 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human CST4 monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 594-conjugated goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Flow Cytometry: Cystatin S Antibody (006) [NBP2-89913]

Flow Cytometry: Cystatin S Antibody (006) [NBP2-89913]

Flow Cytometry: Cystatin S Antibody (006) [NBP2-89913] - Analysis of Human CST4 expression on HeLa cells. The cells were treated, stained with purified anti-Human CST4, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Applications for Cystatin S Antibody (006)

Application
Recommended Usage

ELISA

1:5000-1:10000

Flow Cytometry

1:25-1:100

Immunocytochemistry/ Immunofluorescence

1:20-1:100

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cystatin S

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a type 2 salivary cysteine peptidase inhibitor. The protein is an S-type cystatin, based on its high level of expression in saliva, tears and seminal plasma. The specific role in these fluids is unclear but antibacterial and antiviral activity is present, consistent with a protective function. [provided by RefSeq]

Alternate Names

CST4

Gene Symbol

CST4

Additional Cystatin S Products

Product Documents for Cystatin S Antibody (006)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Cystatin S Antibody (006)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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