Cytochrome c Antibody (6H2.B4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24872

Novus Biologicals, part of Bio-Techne
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NBP2-24872
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Block/Neutralize, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 6H2.B4

Concentration

0.5 mg/ml

View all Cytochrome c Antibodies »

Product Specifications

Immunogen

Rat cytochrome c was used as immunogen to generate 6H2.B4 hybridoma clone (Goshorn et al, 1991). The antibody recognizes an epitope around residue 62 of rat Cytochrome c, based on competitive ELISA results (Goshorn et al. 1991).

Marker

Mitochondria Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Cytochrome c Antibody (6H2.B4)

Immunoprecipitation: Cytochrome c Antibody (6H2.B4) [NBP2-24872]

Immunoprecipitation: Cytochrome c Antibody (6H2.B4) [NBP2-24872]

Immunoprecipitation: Cytochrome c Antibody (6H2.B4) [NBP2-24872] - Immunoprecitation/analysis using cytochrome c antibodies. Two micrograms of cytochrome c antibody clone 6H2.B4 (lane 1) or control IgG antibody (lane 2) were used to immunoprecipitate cytochrome c from 1 x 10^6 HeLa cells. The immunoprecipitated protein was resolved by SDS-PAGE and probed with cytochrome antibody clone 7H8.2C12 .
Immunoprecipitation: Cytochrome c Antibody (6H2.B4) [NBP2-24872]

Immunoprecipitation: Cytochrome c Antibody (6H2.B4) [NBP2-24872]

Immunoprecipitation: Cytochrome c Antibody (6H2.B4) [NBP2-24872] - Cytochrome c was immunoprecipitated from Hela cell extract (1% NP-40) using 2-4 ug 6H2.B4 antibody/1 x107 cell equivalents.

Applications for Cytochrome c Antibody (6H2.B4)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 16540654)

Immunohistochemistry

reported in scientific literature (PMID 28194435)

Immunoprecipitation

2 ug/1X10^6 cells
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 ml PBS and 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cytochrome c

In addition to its well-known role in electron-transport, cytochrome c (CYT) has been identified as a mediator of apoptosis or programmed cell death (1). Cytochrome c translocates from mitochondria into the cytoplasm in the early phases of apoptosis and plays a role in initiation of the apoptotic proteolytic cascade bay activating caspase-3 (2). Cytochrome c has been shown to bind Apaf-1. Apaf-1 has also been shown to activate capase-3 (3).

Alternate Names

CYCS

Gene Symbol

CYCS

Additional Cytochrome c Products

Product Documents for Cytochrome c Antibody (6H2.B4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cytochrome c Antibody (6H2.B4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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