Cytochrome c Antibody (CTC05)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44445
![Western Blot: Cytochrome c Antibody (CTC05) [NBP2-44445]](https://resources.bio-techne.com/images/products/Cytochrome-c-Antibody-CTC05-Western-Blot-NBP2-44445-img0003.jpg "Western Blot: Cytochrome c Antibody (CTC05) [NBP2-44445]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Amphibian, Canine, Drosophila, Equine, Pigeon
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # CTC05
Concentration
0.2 mg/ml
Product Summary for Cytochrome c Antibody (CTC05)
Immunogen
Recombinant cytochrome c protein (Uniprot: P99999)
Reactivity Notes
Frog
Localization
Cytoplasmic
Marker
Mitochondrial Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-11456)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Cytochrome c Antibody (CTC05)
Western Blot: Cytochrome c Antibody (CTC05) [NBP2-44445]
Western Blot: Cytochrome c Antibody (CTC05) [NBP2-44445] - Western Blot Analysis of heart tissue lysate using Cytochrome c Antibody (CTC05).![Immunohistochemistry-Paraffin: Cytochrome c Antibody (CTC05) [NBP2-44445]](https://resources.bio-techne.com/images/products/Cytochrome-c-Antibody-CTC05-Immunohistochemistry-Paraffin-NBP2-44445-img0001.jpg "Immunohistochemistry-Paraffin: Cytochrome c Antibody (CTC05) [NBP2-44445]")
Immunohistochemistry-Paraffin: Cytochrome c Antibody (CTC05) [NBP2-44445]
Immunohistochemistry-Paraffin: Cytochrome c Antibody (CTC05) [NBP2-44445] - Human Heart stained with Cytochrome C Monoclonal Antibody (CTC05).Applications for Cytochrome c Antibody (CTC05)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunohistochemistry-Paraffin
0.25-0.5 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Cytochrome c
Additional Cytochrome c Products
Product Documents for Cytochrome c Antibody (CTC05)
Product Specific Notices for Cytochrome c Antibody (CTC05)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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