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Cytochrome c Antibody (CTC05) - IHC-Prediluted

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48148

Novus Biologicals, part of Bio-Techne
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NBP2-48148

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Amphibian, Canine, Drosophila, Equine, Pigeon

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # CTC05

Format

IHC-Prediluted

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for Cytochrome c Antibody (CTC05) - IHC-Prediluted

Immunogen

Recombinant cytochrome c protein (Uniprot: P99999)

Reactivity Notes

Frog

Localization

Cytoplasmic

Marker

Mitochondrial Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Scientific Data Images for Cytochrome c Antibody (CTC05) - IHC-Prediluted

Immunohistochemistry-Paraffin: Cytochrome c Antibody (CTC05) - IHC-Prediluted [NBP2-48148]

Immunohistochemistry-Paraffin: Cytochrome c Antibody (CTC05) - IHC-Prediluted [NBP2-48148]

Immunohistochemistry-Paraffin: Cytochrome c Antibody (CTC05) - IHC-Prediluted [NBP2-48148] - Human Heart stained with Cytochrome C Monoclonal Antibody (CTC05).

Applications for Cytochrome c Antibody (CTC05) - IHC-Prediluted

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Hu-chromosome location: 1q32.2
Molecular weight of antigen: 90-110kDa
Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Format

IHC-Prediluted

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cytochrome c

In addition to its well-known role in electron-transport, cytochrome c (CYT) has been identified as a mediator of apoptosis or programmed cell death (1). Cytochrome c translocates from mitochondria into the cytoplasm in the early phases of apoptosis and plays a role in initiation of the apoptotic proteolytic cascade bay activating caspase-3 (2). Cytochrome c has been shown to bind Apaf-1. Apaf-1 has also been shown to activate capase-3 (3).

Alternate Names

CYCS

Gene Symbol

CYCS

Additional Cytochrome c Products

Product Documents for Cytochrome c Antibody (CTC05) - IHC-Prediluted

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Cytochrome c Antibody (CTC05) - IHC-Prediluted

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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