Cytochrome P450 17A1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2842

Novus Biologicals, part of Bio-Techne
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NB100-2842
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Canine (100%), Feline (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Cytochrome P450 17A1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-LFKDGDQKLEK corresponding to internal region according to NP_000093.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Cytochrome P450 17A1 Antibody

Western Blot: Cytochrome P450 17A1 Antibody [NB100-2842]

Western Blot: Cytochrome P450 17A1 Antibody [NB100-2842]

Western Blot: Cytochrome P450 17A1 Antibody [NB100-2842] - (0.3ug/ml) staining of human ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody [NB100-2842]

Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody [NB100-2842]

Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody [NB100-2842] - (3.8ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Cytochrome P450 17A1 Antibody

Application
Recommended Usage

Immunohistochemistry

3 - 5 ug/ml

Immunohistochemistry-Paraffin

3 - 5 ug/ml

Peptide ELISA

Detection limit 1:64000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 60 kDa band observed in human ovary lysates (calculated MW of 57.4 kDa band according to NP_000093.1). IHC-P: Human adenal gland shows strong granular staining of the cytoplasm in medulla cells.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cytochrome P450 17A1

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. It has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in this gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17,20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia.

Long Name

Steroid 17-alpha-hydroxylase/17,20 lyase

Alternate Names

CYP17, CYP17A1, CYPXVII, Cytochrome P450-C17, Cytochrome P450c17, EC 1.14.14.19, EC 1.14.14.32, S17AH

Entrez Gene IDs

1586 (Human)

Gene Symbol

CYP17A1

UniProt

P05093 (Human)

Additional Cytochrome P450 17A1 Products

Product Documents for Cytochrome P450 17A1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Cytochrome P450 17A1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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