Cytochrome P450 1A2 Antibody (8B2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15169

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15169
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8B2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Cytochrome P450 1A2 Antibodies »

Product Summary for Cytochrome P450 1A2 Antibody (8B2)

Immunogen

A synthesized peptide derived from human Cytochrome P450 1A2. UniProt ID (P05177).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cytochrome P450 1A2 Antibody (8B2)

Western Blot: Cytochrome P450 1A2 Antibody (8B2) [NBP3-15169]

Western Blot: Cytochrome P450 1A2 Antibody (8B2) [NBP3-15169]

Western Blot: Cytochrome P450 1A2 Antibody (8B2) [NBP3-15169] - Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, L02 whole cell lysate. All lanes: Cytochrome P450 1A2 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 59 KDa. Observed band size: 59 KDa.
Immunohistochemistry: Cytochrome P450 1A2 Antibody (8B2) [NBP3-15169]

Immunohistochemistry: Cytochrome P450 1A2 Antibody (8B2) [NBP3-15169]

Immunohistochemistry: Cytochrome P450 1A2 Antibody (8B2) [NBP3-15169] - IHC image of Cytochrome P450 1A2 antibody diluted at 1:100 and staining in paraffin-embedded human kidney tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Applications for Cytochrome P450 1A2 Antibody (8B2)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 150mM NaCl, and 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Cytochrome P450 1A2

In animals, P450 enzymes serve two major functions: (1) biosynthesis of steroids, fatty acids, and bile acids; (2) metabolism of endogenous and a wide variety of exogenous substrates, such as toxins and drugs. The four major families involved in drug metabolism are CYP 1,2,3, and 4. CYP1A2 is an aryl hydrocarbon hydroxylase involved in the metabolism of endogenous compounds and xenobiotics. It is one of the major drug metabolising enzymes in humans.

Long Name

Cytochrome P450 1A2

Alternate Names

CYP1A2, CYPIA2, Cytochrome P(3)450, Cytochrome P450 4, Cytochrome P450-P3

Gene Symbol

CYP1A2

Additional Cytochrome P450 1A2 Products

Product Documents for Cytochrome P450 1A2 Antibody (8B2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cytochrome P450 1A2 Antibody (8B2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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