Cytochrome P450 1A2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97780

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Cytochrome P450 1A2 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-HSENYKDNGGLIPEEK corresponding to internal region according to Uniprot Mouse NP_034123.1.

Specificity

This antibody is not expected to cross-react to the similar P450 1A1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Cytochrome P450 1A2 Antibody

Western Blot: Cytochrome P450 1A2 Antibody [NBP1-97780]

Western Blot: Cytochrome P450 1A2 Antibody [NBP1-97780]

Western Blot: Cytochrome P450 1A2 Antibody [NBP1-97780] - staining of Human Liver (A) and (1ug/ml) Lung (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: Cytochrome P450 1A2 Antibody [NBP1-97780]

Western Blot: Cytochrome P450 1A2 Antibody [NBP1-97780]

Western Blot: Cytochrome P450 1A2 Antibody [NBP1-97780] - (1ug/ml) Analysis of fetal Mouse Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Cytochrome P450 1A2 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 60 kDa band observed in mouse fetal liver lysates (calculated MW of 58.2 kDa band according to NP_034123.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cytochrome P450 1A2

In animals, P450 enzymes serve two major functions: (1) biosynthesis of steroids, fatty acids, and bile acids; (2) metabolism of endogenous and a wide variety of exogenous substrates, such as toxins and drugs. The four major families involved in drug metabolism are CYP 1,2,3, and 4. CYP1A2 is an aryl hydrocarbon hydroxylase involved in the metabolism of endogenous compounds and xenobiotics. It is one of the major drug metabolising enzymes in humans.

Long Name

Cytochrome P450 1A2

Alternate Names

CYP1A2, CYPIA2, Cytochrome P(3)450, Cytochrome P450 4, Cytochrome P450-P3

Entrez Gene IDs

1544 (Human); 13077 (Mouse); 24297 (Rat)

Gene Symbol

CYP1A2

UniProt

NP_034123.1 (Mouse)

Additional Cytochrome P450 1A2 Products

Product Documents for Cytochrome P450 1A2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Cytochrome P450 1A2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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