Cytohesin-1 Antibody (6G11)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22513

Novus Biologicals, part of Bio-Techne
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NBP2-22513
NBP2-22513-0.025mg
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 6G11

Concentration

1 mg/ml

View all Cytohesin-1 Antibodies »

Product Summary for Cytohesin-1 Antibody (6G11)

Immunogen

Purified, His-tagged cytohesin-1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Cytohesin-1 Antibody (6G11)

Immunocytochemistry/ Immunofluorescence: Cytohesin-1 Antibody (6G11) [NBP2-22513]

Immunocytochemistry/ Immunofluorescence: Cytohesin-1 Antibody (6G11) [NBP2-22513]

Immunocytochemistry/Immunofluorescence: Cytohesin-1 Antibody (6G11) [NBP2-22513] - Analysis of Cytohesin 1,2 in NIH-3T3 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Cytohesin 1,2 monoclonal antibody at a dilution of 1:100 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Cytohesin 1,2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.
Immunohistochemistry: Cytohesin-1 Antibody (6G11) [NBP2-22513]

Immunohistochemistry: Cytohesin-1 Antibody (6G11) [NBP2-22513]

Immunohistochemistry: Cytohesin-1 Antibody (6G11) [NBP2-22513] - Normal biopsies of deparaffinized human placenta tissue.
Immunocytochemistry/ Immunofluorescence: Cytohesin-1 Antibody (6G11) [NBP2-22513]

Immunocytochemistry/ Immunofluorescence: Cytohesin-1 Antibody (6G11) [NBP2-22513]

Immunocytochemistry/Immunofluorescence: Cytohesin-1 Antibody (6G11) [NBP2-22513] - Analysis of Cytohesin 1,2 in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Cytohesin 1,2 monoclonal antibody at a dilution of 1:100 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Cytohesin 1,2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.
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Applications for Cytohesin-1 Antibody (6G11)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:200 - 500

Immunohistochemistry-Paraffin

1:200 - 500

Immunoprecipitation

1:10 - 1:500

Western Blot

1.0 ug/ml
Application Notes
WB: Detects an approx. 48 kDa band representing both cytohesin-1 and cytohesin-2. IP: Only precipitates cytohesin-2.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

PEG purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cytohesin-1

Pleckstrin homology, Sec7 and coiled/coil domains 1 (PSCD1) is a member of the PSCD family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. The PSCD1 is highly expressed in natural killer and peripheral T cells, and regulates the adhesiveness of integrins at the plasma membrane of lymphocytes. PSCD1 protein is 83% homologous to PSCD2.

Alternate Names

B2-1, CYTH1, Cytohesin1, PSCD1, SEC7

Entrez Gene IDs

9267 (Human)

Gene Symbol

CYTH1

UniProt

Q15438

Additional Cytohesin-1 Products

Product Documents for Cytohesin-1 Antibody (6G11)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Cytohesin-1 Antibody (6G11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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