Cytohesin 2 Antibody (CYT2-21) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB120-12421
Key Product Details
Species Reactivity
Human, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CYT2-21
Format
BSA Free
Concentration
2.0 mg/ml
Product Specifications
Immunogen
Recombinant full length protein (Human).
Reactivity Notes
Cross-reacts with Human and Rat.
Localization
Plasma membrane
Specificity
does not cross-react with other members of the cytohesin family.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Cytohesin 2 Antibody (CYT2-21) - BSA Free
![Western Blot: Cytohesin 2 Antibody (CYT2-21)BSA Free [NB120-12421]](https://resources.bio-techne.com/images/products/Cytohesin-2-Antibody-CYT2-21-BSA-Free-Western-Blot-NB120-12421-img0003.jpg "Western Blot: Cytohesin 2 Antibody (CYT2-21)BSA Free [NB120-12421]")
Western Blot: Cytohesin 2 Antibody (CYT2-21)BSA Free [NB120-12421]
Western Blot: Cytohesin 2 Antibody (CYT2-21) - BSA Free [NB120-12421] - Whole extract of Rat brain were separated on SDS-PAGE and probed with Monoclonal Anti-Cytohesin 2 antibody produced in Mouse, Clone: CYT2-21. The antibody was developed using 1:1000 Anti-Mouse IgG (Fab specific)-Biotin antibody produced in Goat and 1:10,000 ExtrAvidin-Alkaline.Lane:1. 5 ug/mL antibody2. 10 ug/mL antibody3. Negative ControlApplications for Cytohesin 2 Antibody (CYT2-21) - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
1:10-1:2000
Western Blot
5 - 10 ug/ml
Application Notes
ELISA: Use at an assay dependent dilution. ICC: Use at an assay dependent dilution. Western Blot: Use at a concentration of 5 - 10 ug/ml, this concentration is determined using a whole extract of rat brain. Predicted molecular weight: 40 kDa. Optimal dilutions/concentrations should be determined by the end user.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS (pH 7.4)
Format
BSA Free
Preservative
0.9% Sodium Azide
Concentration
2.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Cytohesin 2
Arf is involved in intracellular protein traffic to and within the Golgi complex. Arf has a number of disparate activities including maintenance of organelle integrity, assembly of coat proteins, as a co-factor for cholera toxin and as an activator of phospholipase D.
Like other small GTPases, Arf is found to be active when bound to GTP and inactive when bound to GDP. Arf's activation is dependent upon guanine nucleotide exchange factors (GEFs) which increase the rate of exchange of bound GDP with GTP. All GEFs have a highly conserved Sec7 domain. GEF activity lies in the Sec7 domain and this activity has been shown to be inhibited by the fungal metabolite brefeldin-A (BFA). A small group of GEFs which are insensitive to brefeldin-A (BFA) include cytohesin-1 (B2-1), cytohesin-2 (ARNO), cytohesin-3 (ARNO3), and cytohesin-4. All cytohesins function in the cell periphery and contain a pleckstrin homology (PH) domain. The PH domain has been shown to interact with phosphatidylinositol 3,4,5-triphosphate and is believed to promote membrane targeting of the cytohesins. Recruitment of the cytohesins to the membranes can occur in response to tyrosine kinase receptor activation. This response appears to require the activation of phosphatidylinositol 3-kinase (PI 3-kinase).
Long Name
Cytohesin-2
Alternate Names
ARF exchange factor, ARNO, CYTH2, Protein ARNO, PSCD2, PSCD2L
Gene Symbol
CYTH2
Additional Cytohesin 2 Products
Product Documents for Cytohesin 2 Antibody (CYT2-21) - BSA Free
Product Specific Notices for Cytohesin 2 Antibody (CYT2-21) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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