Cytokeratin 1 Antibody (LHK1) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2756

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human, Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # LHK1

Format

BSA Free

Concentration

1.0 mg/ml

View all Cytokeratin 1 Antibodies »

Product Specifications

Immunogen

C-terminal peptide sequence of human Cytokeratin 1 [UniProt# P04264]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cytokeratin 1 Antibody (LHK1) - BSA Free

Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (LHK1) - BSA Free [NB100-2756]

Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (LHK1) - BSA Free [NB100-2756]

Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (LHK1) [NB100-2756] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human breast cancer using 5ug/ml conc. of Cytokeratin 1 antibody (clone LHK1) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) was performed using Aperio AT2 (Leica Biosystems). A membrane-cytoplasmic staining of Cytokeratin 1 was observed in the cancer cells with signal localized more to the cell membranes. Tumor cores showed a decreased staining and tumor stroma was mostly negative for Cytokeratin 1.
Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (LHK1) - BSA Free [NB100-2756]

Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (LHK1) - BSA Free [NB100-2756]

Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (LHK1) [NB100-2756] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human breast cancer using 5ug/ml conc. of Cytokeratin 1 antibody (clone LHK1) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) was performed using Aperio AT2 (Leica Biosystems). A membrane-cytoplasmic staining of Cytokeratin 1 was observed in the cancer cells with signal localized more to the cell membranes. Tumor stroma was mostly negative for Cytokeratin 1. Staining was performed by Histowiz.
Western Blot: Cytokeratin 1 Antibody (LHK1)BSA Free [NB100-2756]

Western Blot: Cytokeratin 1 Antibody (LHK1)BSA Free [NB100-2756]

Western Blot: Cytokeratin 1 Antibody (LHK1) [NB100-2756] - Analysis of Cytokeratin 1 in Caco-2.
View 1 more images

Applications for Cytokeratin 1 Antibody (LHK1) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

5 ug/ml

Immunohistochemistry

2-10 ug/ml

Immunohistochemistry-Frozen

5-10 ug/ml

Immunohistochemistry-Paraffin

2-5 ug/ml

Western Blot

0.5-2 ug/ml
Application Notes
This Cytokeratin 1 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-frozen sections, Immunohistochemistry-paraffin sections and Western blot.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cytokeratin 1

Cytokeratin 1 is a heterotetramer of two type I as well as two type II keratins localized in the cell membrane and is generally associated with keratin-10 (KRT10). It has been suggested to regulate the activity of kinases such as PKC/SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein kinase C (RACK1/GNB2L1). Cytokeratin 1 and its heterodimer partner KRT10 are the major constituents of 4 intermediate filament cytoskeleton in suprabasal epidermis/keratinocytes, and their upregulation precedes expression of filaggrin and cornified envelope proteins. Covalent crosslinking of cytokeratin 1 but not of KRT10 to these proteins, in addition to a complex series of lipids, ultimately forms the cornified/lipid envelope, that constitutes the epidermal barrier together with Langerhans cells. Defects in cytokeratin 1 have been linked to epidermolytic hyperkeratosis (EHK), ichthyosis hystrix Curth-Macklin type (IHCM), palmoplantar keratoderma non-epidermolytic (NEPPK), ichthyosis annular epidermolytic (AEI), palmoplantar keratoderma striate type 3 (SPPK3).

Alternate Names

CK1, CK-1, cytokeratin 1, Cytokeratin-1, cytokeratin-1,67 kDa cytokeratin, EHK, EHK1, epidermolytic hyperkeratosis 1, EPPK, Hair alpha protein, K1, keratin 1, keratin, type II cytoskeletal 1, Keratin-1, KRT1A, KRTA, NEPPK, Type-II keratin Kb1

Entrez Gene IDs

3848 (Human)

Gene Symbol

KRT1

UniProt

P04264 (Human)

Additional Cytokeratin 1 Products

Product Documents for Cytokeratin 1 Antibody (LHK1) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cytokeratin 1 Antibody (LHK1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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