Cytokeratin 1 Antibody (LHK1) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-2756
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Rat
Cited:
Human, Rat
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # LHK1
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
C-terminal peptide sequence of human Cytokeratin 1 [UniProt# P04264]
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Theoretical MW
67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Cytokeratin 1 Antibody (LHK1) - BSA Free
Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (LHK1) - BSA Free [NB100-2756]
Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (LHK1) [NB100-2756] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human breast cancer using 5ug/ml conc. of Cytokeratin 1 antibody (clone LHK1) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) was performed using Aperio AT2 (Leica Biosystems). A membrane-cytoplasmic staining of Cytokeratin 1 was observed in the cancer cells with signal localized more to the cell membranes. Tumor cores showed a decreased staining and tumor stroma was mostly negative for Cytokeratin 1.Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (LHK1) - BSA Free [NB100-2756]
Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (LHK1) [NB100-2756] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human breast cancer using 5ug/ml conc. of Cytokeratin 1 antibody (clone LHK1) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) was performed using Aperio AT2 (Leica Biosystems). A membrane-cytoplasmic staining of Cytokeratin 1 was observed in the cancer cells with signal localized more to the cell membranes. Tumor stroma was mostly negative for Cytokeratin 1. Staining was performed by Histowiz.Western Blot: Cytokeratin 1 Antibody (LHK1)BSA Free [NB100-2756]
Western Blot: Cytokeratin 1 Antibody (LHK1) [NB100-2756] - Analysis of Cytokeratin 1 in Caco-2.Applications for Cytokeratin 1 Antibody (LHK1) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
5 ug/ml
Immunohistochemistry
2-10 ug/ml
Immunohistochemistry-Frozen
5-10 ug/ml
Immunohistochemistry-Paraffin
2-5 ug/ml
Western Blot
0.5-2 ug/ml
Application Notes
This Cytokeratin 1 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-frozen sections, Immunohistochemistry-paraffin sections and Western blot.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Cytokeratin 1
Alternate Names
CK1, CK-1, cytokeratin 1, Cytokeratin-1, cytokeratin-1,67 kDa cytokeratin, EHK, EHK1, epidermolytic hyperkeratosis 1, EPPK, Hair alpha protein, K1, keratin 1, keratin, type II cytoskeletal 1, Keratin-1, KRT1A, KRTA, NEPPK, Type-II keratin Kb1
Entrez Gene IDs
3848 (Human)
Gene Symbol
KRT1
UniProt
Additional Cytokeratin 1 Products
Product Documents for Cytokeratin 1 Antibody (LHK1) - BSA Free
Product Specific Notices for Cytokeratin 1 Antibody (LHK1) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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