Cytokeratin 14 Antibody (KRT14/8260R)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23907

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-23907-100ug
NBP3-23907-20ug
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # KRT14/8260R

Concentration

0.2 mg/ml

View all Cytokeratin 14 Antibodies »

Product Specifications

Immunogen

Recombinant human Cytokeratin 14 fragment (around aa272-472) (exact sequence is proprietary)

Localization

Cytoplasm.

Marker

Squamous Cell Marker

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

Store at 2 to 8C. Non-hazardous. No MSDS required.

Scientific Data Images for Cytokeratin 14 Antibody (KRT14/8260R)

Cytokeratin 14 Antibody (KRT14/8260R) Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (KRT14/8260R) [NBP3-23907] -

Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (KRT14/8260R) [NBP3-23907] -

Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (KRT14/8260R) [NBP3-23907] - Formalin-fixed, paraffin-embedded human prostate stained with Cytokeratin 14 Antibody (KRT14/8260R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Applications for Cytokeratin 14 Antibody (KRT14/8260R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cytokeratin 14

Keratins are cytoplasmic intermediate filament proteins expressed by epithelial cells. Cytokeratin 14 (CK-14) is a 50-kDa keratin expressed in abundance in human epidermal cells (1). During anagen, cell proliferation in the germinative matrix of the hair follicle gives rise to the fiber and inner root sheath. The transition from anagen to telogen is marked by downregulation of hair cortical specific keratins and the appearance of CK-14 in the epithelial sac to which the telogen hair fiber is anchored (2). A family with recessive epidermolysis bullosa simplex lacked expression of the basal cell keratin 14. The patients had severe generalized skin blistering that improved slightly with age. The basal cells of the patients did not express keratin 14 and contained no keratin intermediate filaments. The expression of keratin 5, the obligate copolymer of keratin 14, was slightly reduced (3).

Alternate Names

CK14, KRT14

Gene Symbol

KRT14

Additional Cytokeratin 14 Products

Product Documents for Cytokeratin 14 Antibody (KRT14/8260R)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cytokeratin 14 Antibody (KRT14/8260R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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