Cytokeratin 14 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31326
Key Product Details
Species Reactivity
Validated:
Human, Rat
Predicted:
Bovine (96%), Canine (96%), Mouse (90%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human Cytokeratin 14. The exact sequence is proprietary.
Localization
Cytoplasm, Nucleus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Cytokeratin 14 Antibody
![Immunocytochemistry/ Immunofluorescence: Cytokeratin 14 Antibody [NBP1-31326]](https://resources.bio-techne.com/images/products/Cytokeratin-14-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31326-img0009.jpg "Immunocytochemistry/ Immunofluorescence: Cytokeratin 14 Antibody [NBP1-31326]")
Immunocytochemistry/ Immunofluorescence: Cytokeratin 14 Antibody [NBP1-31326]
Immunocytochemistry/Immunofluorescence: Cytokeratin 14 Antibody [NBP1-31326] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cytokeratin 14 protein stained by Cytokeratin 14 antibody diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody [NBP1-31326]](https://resources.bio-techne.com/images/products/Cytokeratin-14-Antibody-Immunohistochemistry-Paraffin-NBP1-31326-img0004.jpg "Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody [NBP1-31326]")
Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody [NBP1-31326]
Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody [NBP1-31326] - Lung CA, using Cytokeratin 14 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: Cytokeratin 14 Antibody [NBP1-31326] -
Western Blot: Cytokeratin 14 Antibody [NBP1-31326] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 14 antibody (NBP1-31326) diluted at 1:6000.Applications for Cytokeratin 14 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:1000-1:10000
Application Notes
IHC-FR assay dependent
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Cytokeratin 14
Additional Cytokeratin 14 Products
Product Specific Notices for Cytokeratin 14 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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