Cytokeratin 15 Antibody (ST04-85)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67525
Recombinant Monoclonal Antibody.
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # ST04-85
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within Human Cytokeratin 15 aa 410-455 / 456. (SwissProt: P19012 Human; SwissProt: Q61414 Mouse; SwissProt: Q6IFV3 Rat)
Localization
Intermediate filament, cytosol, extracellular exosome, nucleus.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Cytokeratin 15 Antibody (ST04-85)
Western Blot: Cytokeratin 15 Antibody (ST04-85) [NBP2-67525]
Western Blot: Cytokeratin 15 Antibody (ST04-85) [NBP2-67525] - Analysis of Cytokeratin 15 on A431 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody (ST04-85) [NBP2-67525]
Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody (ST04-85) [NBP2-67525] - Analysis of paraffin-embedded human skin tissue with Rabbit anti-Cytokeratin 15 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody (ST04-85) [NBP2-67525]
Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody (ST04-85) [NBP2-67525] - Analysis of paraffin-embedded mouse skin tissue using anti-Cytokeratin 15 antibody. Counter stained with hematoxylin.Applications for Cytokeratin 15 Antibody (ST04-85)
Application
Recommended Usage
Flow Cytometry
1:50-1:100
Immunocytochemistry/ Immunofluorescence
1:400-1:500
Immunohistochemistry-Paraffin
1:50-1:400
Western Blot
1:500-1:2000
Application Notes
Use in ICC/IF reported in scientific literature (PMID:35664254) .
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Cytokeratin 15
Alternate Names
CK15, CK-15, cytokeratin 15, cytokeratin-15, K15keratin-15, basic, K1CO, keratin 15, keratin, type I cytoskeletal 15, Keratin-15, keratin-15, beta, KRTB, type I cytoskeletal 15
Gene Symbol
KRT15
Additional Cytokeratin 15 Products
Product Documents for Cytokeratin 15 Antibody (ST04-85)
Product Specific Notices for Cytokeratin 15 Antibody (ST04-85)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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