Cytokeratin 17 Antibody (9F3) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-42619

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 9F3

Format

BSA Free

Concentration

1 mg/ml

View all Cytokeratin 17 Antibodies »

Product Summary for Cytokeratin 17 Antibody (9F3) - BSA Free

Immunogen

Recombinant human Cytokeratin 17 (1-432aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Cytokeratin 17 Antibody (9F3) - BSA Free

Western Blot: Cytokeratin 17 Antibody (9F3)BSA Free [NBP2-42619]

Western Blot: Cytokeratin 17 Antibody (9F3)BSA Free [NBP2-42619]

Western Blot: Cytokeratin 17 Antibody (9F3) [NBP2-42619] - Western Blot: The Recombinant Human Cytokeratin 17 (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cytokeratin 17 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human Cytokeratin 17 Lane 2.: HeLa cell lysate Lane 3.: A431 cell lysate
Immunocytochemistry/ Immunofluorescence: Cytokeratin 17 Antibody (9F3) - BSA Free [NBP2-42619]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 17 Antibody (9F3) - BSA Free [NBP2-42619]

Immunocytochemistry/Immunofluorescence: Cytokeratin 17 Antibody (9F3) [NBP2-42619] - Analysis of KRT17 in A431 cells. The cell was stained at (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Flow Cytometry: Cytokeratin 17 Antibody (9F3) - BSA Free [NBP2-42619]

Flow Cytometry: Cytokeratin 17 Antibody (9F3) - BSA Free [NBP2-42619]

Flow Cytometry: Cytokeratin 17 Antibody (9F3) [NBP2-42619] - Flow cytometry analysis of Cytokeratin 17 in A431 cells. The cell was stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
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Applications for Cytokeratin 17 Antibody (9F3) - BSA Free

Application
Recommended Usage

Flow Cytometry

2-5ug

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cytokeratin 17

Among the members of the cytokeratin (CK) subfamily of intermediate filament (IF) proteins, CK 17 is normally expressed in the basal cells of complex epithelia but not in stratified or simple epithelia. It has an unusual expression pattern in normal and diseased states and has potential importance in tumor diagnosis. CK 17 synthesis seems to be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial "stem cells" (1). It has been shown that CK 17 regulates cell growth through binding to the adaptor protein 14-3-3sigma. These findings reveal a new and unexpected role for the intermediate filament cytoskeleton in influencing cell growth and size by regulating protein synthesis (2). CK 17 is expressed in the nail bed, hair follicle, sebaceous glands, and other epidermal appendages (3).

Long Name

Cytokeratin 17/KRT17

Alternate Names

CK-17, cytokeratin-17, K17, keratin 17, type I, keratin, type I cytoskeletal 17, PCHC1

Gene Symbol

KRT17

UniProt

NP_000413 (Human)

Additional Cytokeratin 17 Products

Product Documents for Cytokeratin 17 Antibody (9F3) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Cytokeratin 17 Antibody (9F3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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