Cytokeratin 17 Antibody (E3 (same as Ks17.E3)) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33188

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Unconjugated

NBP2-29421

Alexa Fluor 350

NBP2-33188AF350

Alexa Fluor 405

NBP2-33188AF405

Alexa Fluor 488

NBP2-33188AF488

Alexa Fluor 532

NBP2-33188AF532

Alexa Fluor 594

NBP2-33188AF594

Alexa Fluor 647

NBP2-33188AF647

Alexa Fluor 700

NBP2-33188AF700

Alexa Fluor 750

NBP2-33188AF750

Allophycocyanin

NBP2-33188APC

Biotin

NBP2-33188B

DyLight 350

NBP2-33188UV

DyLight 405

NBP2-33188V

DyLight 488

NBP2-33188G

DyLight 550

NBP2-33188R

DyLight 594

NBP2-33188DL594

DyLight 650

NBP2-33188C

DyLight 680

NBP2-33188FR

DyLight 755

NBP2-33188IR

FITC

NBP2-33188F

HRP

NBP2-33188H

Janelia Fluor 549

NBP2-33188JF549

Janelia Fluor 646

NBP2-33188JF646

mFluor Violet 450 SE

NBP2-33188MFV450

mFluor Violet 500 SE

NBP2-33188MFV500

mFluor Violet 610 SE

NBP2-33188MFV610

PE

NBP2-33188PE

PerCP

NBP2-33188PCP

Forumulation

Catalog #

IHC-Prediluted

NBP2-44425

Product Summary
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human, Rat, Porcine, Bovine, Goat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # E3 (same as Ks17.E3)

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all Cytokeratin 17 Antibodies »

Product Summary for Cytokeratin 17 Antibody (E3 (same as Ks17.E3)) - Azide and BSA Free

Immunogen

Cytoskeletal fraction of rat colon epithelium (Uniprot: Q04695)

Localization

Cytoplasmic

Specificity

Cytokeratin 17 (CK17) is normally expressed in the basal cells of complex epithelia but not in stratified or simple epithelia. Antibody to CK17 is an excellent tool to distinguish myoepithelial cells from luminal epithelium of various glands such as mammary, sweat and salivary. CK17 is expressed in epithelial cells of various origins, such as bronchial epithelial cells and skin appendages. It may be considered as 'epithelial stem cell' marker because CK17 Ab marks basal cell differentiation. CK17 is expressed in SCLC much higher than in LADC. Eighty-five percent of the triple negative breast carcinomas immunoreact with basal cytokeratins including anti-CK17. Also important is that cases of triple negative breast carcinoma with expression of CK17 show an aggressive clinical course. The histologic differentiation of ampullary cancer, intestinal vs. pancreatobiliary, is very important for treatment. Usually anti-CK17 and anti-MUC1 immunoreactivity represents pancreatobiliary subtype whereas anti-MUC2 and anti-CDX-2 positivity defines intestinal subtype.

Marker

Basal Epithelial Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-29421).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Cytokeratin 17 Antibody (E3 (same as Ks17.E3)) - Azide and BSA Free

Western Blot: Cytokeratin 17 Antibody (E3 (same as Ks17.E3))Azide and BSA Free [NBP2-33188]

Western Blot: Cytokeratin 17 Antibody (E3 (same as Ks17.E3)) - Azide and BSA Free [NBP2-33188] - Analysis of Cytokeratin 17 in human HeLa lysate using Cytokeratin 17 E3 antibody at 3 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Immunohistochemistry-Paraffin: Cytokeratin 17 Antibody (E3 (same as Ks17.E3)) - Azide and BSA Free [NBP2-33188]

Immunohistochemistry-Paraffin: Cytokeratin 17 Antibody (E3 (same as Ks17.E3)) - Azide and BSA Free [NBP2-33188] - Formalin-fixed paraffin-embedded human Bladder Carcinoma stained with CK17 Monoclonal Antibody (E3)

Immunohistochemistry-Paraffin: Cytokeratin 17 Antibody (E3 (same as Ks17.E3)) - Azide and BSA Free [NBP2-33188]

Immunohistochemistry-Paraffin: Cytokeratin 17 Antibody (E3 (same as Ks17.E3)) - Azide and BSA Free [NBP2-33188] - Formalin-fixed, paraffin-embedded human cervical carcinoma stained with CK17 Monoclonal Antibody (E3).

Applications for Cytokeratin 17 Antibody (E3 (same as Ks17.E3)) - Azide and BSA Free

Application

Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Immunohistochemistry (Frozen & formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Cytokeratin 17

Among the members of the cytokeratin (CK) subfamily of intermediate filament (IF) proteins, CK 17 is normally expressed in the basal cells of complex epithelia but not in stratified or simple epithelia. It has an unusual expression pattern in normal and diseased states and has potential importance in tumor diagnosis. CK 17 synthesis seems to be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial "stem cells" (1). It has been shown that CK 17 regulates cell growth through binding to the adaptor protein 14-3-3sigma. These findings reveal a new and unexpected role for the intermediate filament cytoskeleton in influencing cell growth and size by regulating protein synthesis (2). CK 17 is expressed in the nail bed, hair follicle, sebaceous glands, and other epidermal appendages (3).

Long Name

Cytokeratin 17/KRT17

Alternate Names

CK-17, cytokeratin-17, K17, keratin 17, type I, keratin, type I cytoskeletal 17, PCHC1

Gene Symbol

KRT17

Additional Cytokeratin 17 Products

Product Documents for Cytokeratin 17 Antibody (E3 (same as Ks17.E3)) - Azide and BSA Free

Product Datasheets

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COA

SDS

Product Specific Notices for Cytokeratin 17 Antibody (E3 (same as Ks17.E3)) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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