Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) - IHC-Prediluted
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48075
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) - IHC-Prediluted [NBP2-48075]](https://resources.bio-techne.com/images/products/Cytokeratin-18-Antibody-C-04-same-as-Ks18-04-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48075-img0002.jpg "Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) - IHC-Prediluted [NBP2-48075]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken (Negative), Equine, Feline, Ferret, Goat, Hamster, Monkey, Sheep, Xenopus (Negative)
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # C-04 (same as Ks18.04)
Format
IHC-Prediluted
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) - IHC-Prediluted
Immunogen
Cytoskeletal preparation extracted from the human vulvar squamous carcinoma cell line A431
Reactivity Notes
Marmoset Monkey. Does not react with Chicken or Xenopus.
Localization
Cytoplasmic
Specificity
This monoclonal antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
Scientific Data Images for Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) - IHC-Prediluted
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) - IHC-Prediluted [NBP2-48075]
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) - IHC-Prediluted [NBP2-48075] - Human Thyroid Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (C-04).Applications for Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) - IHC-Prediluted
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Visualization of the antigen present in tissue sections is accomplished in a multi-step immunohistochemical staining process, in conjunction with a horseradish peroxidase (HRP) or alkaline phosphatase (AP) linked detection system. Positive control: Tonsil. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Format
IHC-Prediluted
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Cytokeratin 18
Alternate Names
CK-18, CYK18, Keratin 18, KRT18
Gene Symbol
KRT18
Additional Cytokeratin 18 Products
Product Documents for Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) - IHC-Prediluted
Product Specific Notices for Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) - IHC-Prediluted
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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