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Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) [Alexa Fluor® 350]

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11485AF350

Novus Biologicals, part of Bio-Techne
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NBP3-11485AF350

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken (Negative), Equine, Feline, Ferret, Goat, Hamster, Monkey, Sheep, Xenopus (Negative)

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Alexa Fluor 350 (Excitation = 346 nm, Emission = 442 nm)

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # C-04 (same as Ks18.04)

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) [Alexa Fluor® 350]

Immunogen

Cytoskeletal preparation extracted from the human vulvar squamous carcinoma cell line A431

Reactivity Notes

Marmoset Monkey. Does not react with Chicken or Xenopus.

Localization

Cytoplasmic

Specificity

This monoclonal antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) [Alexa Fluor® 350]

Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) [Alexa Fluor® 350]

Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) [Alexa Fluor® 350] [NBP3-11485AF350] -

Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) [Alexa Fluor® 350] [NBP3-11485AF350] - Vial of Alexa Fluor 350 conjugated antibody. Alexa Fluor 350 is optimally excited at 346 nm by the UV laser (350 or 355 nm) and has an emission maximum of 442 nm.

Applications for Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) [Alexa Fluor® 350]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Cytokeratin 18

Cytokeratins are cytoskeletal intermediate filament proteins that are classified as type I (acidic) and type II (neutral to basic) and associate into heterotetramers.

Alternate Names

CK-18, CYK18, Keratin 18, KRT18

Gene Symbol

KRT18

Additional Cytokeratin 18 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) [Alexa Fluor® 350]



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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