Cytokeratin 18 Antibody (C-04) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB500-306
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Mammal
Cited:
Human, Mouse
Applications
Validated:
CyTOF-ready, ELISA, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
ELISA, IF/IHC, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # C-04
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Cytoskeleton preparation of epidermal carcinoma cell line A431.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Specificity
The antibody C-04 reacts with Cytokeratin 18 (45 kDa), a member of intermediate filaments subfamily represented in epithelial tissues.
Marker
Epithelial Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Cytokeratin 18 Antibody (C-04) - BSA Free
Western Blot: Cytokeratin 18 Antibody (C-04)BSA Free [NB500-306]
Western Blot: Cytokeratin 18 Antibody (C-04) [NB500-306] - Analysis of cytokeratin 18 in HeLa cells with anti-cytokeratin 18 (C-04) biotin.Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (C-04) - BSA Free [NB500-306]
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (C-04) [NB500-306] - Immunohistochemistry staining of human kidney (paraffin-embedded sections) with anti-cytokeratin 18 (C-04). Image using the Biotin format of this antibody.Flow Cytometry: Cytokeratin 18 Antibody (C-04) - BSA Free [NB500-306]
Flow Cytometry: Cytokeratin 18 Antibody (C-04) [NB500-306] - Intracellular flow cytometry analysis of cytokeratin expression in HeLa human cervix carcinoma cell line using anti-cytokeratin 18 (C-04) FITC.Applications for Cytokeratin 18 Antibody (C-04) - BSA Free
Application
Recommended Usage
ELISA
1:2000
Flow (Intracellular)
1-4 ug/ml
Flow Cytometry
1-4 ug/ml
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
10 ug/ml
Immunoprecipitation
1:50
Western Blot
1-2 ug/ml
Application Notes
Predicted molecular weight: 45 kDa. Western Blot - Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Application note: Non-reducing conditions. SDS-PAGE (10% separating gel) ELISA - The antibody C-04 has been tested as the capture antibody in a sandwich ELISA for analysis of Cytokeratin 18 in combination with antibody DA-7 and with the antibody DC-10. This antibody is CyTOF ready.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4)
Format
BSA Free
Preservative
0.9% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Cytokeratin 18
Alternate Names
CK-18, CYK18, Keratin 18, KRT18
Gene Symbol
KRT18
Additional Cytokeratin 18 Products
Product Documents for Cytokeratin 18 Antibody (C-04) - BSA Free
Product Specific Notices for Cytokeratin 18 Antibody (C-04) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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