Cytokeratin 18 Antibody (RGE53) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97715

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Canine, Chicken, Guinea Pig, Hamster, Rabbit, Zebrafish

Cited:

Porcine, Canine, Guinea Pig

Applications

Validated:

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Cited:

Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # RGE53

Format

BSA Free

Concentration

1 mg/ml

View all Cytokeratin 18 Antibodies »

Product Specifications

Immunogen

Derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal preparation of HeLa cells.

Reactivity Notes

Use in Guinea Pig reported in scientific literature (PMID:10.1186/s40850-021-00074-w). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody reacts exclusively with Cytokeratin 18 which is present in glandular epithelial cells of the digestive, respiratory, and urogenital tracts, endocrine and exocrine cells and mesothelial cells, as well as adenocarcinomas originating from them.

Marker

Epithelial Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

The antibody is shipped at ambient temperature and may be stored at 4C. For prolonged storage prepare appropriate aliquots and store at or below -20C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Scientific Data Images for Cytokeratin 18 Antibody (RGE53) - BSA Free

Immunocytochemistry/ Immunofluorescence: Cytokeratin 18 Antibody (RGE53) - BSA Free [NBP1-97715]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 18 Antibody (RGE53) - BSA Free [NBP1-97715]

Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Staining of 1 month old zebrafish embryo
Immunohistochemistry: Cytokeratin 18 Antibody (RGE53) - BSA Free [NBP1-97715]

Immunohistochemistry: Cytokeratin 18 Antibody (RGE53) - BSA Free [NBP1-97715]

Immunohistochemistry: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Immunofluorescence staining of human colon epithelium.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 18 Antibody (RGE53) - BSA Free [NBP1-97715]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 18 Antibody (RGE53) - BSA Free [NBP1-97715]

Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Staining of a 2 days old zebrafish embryo. Left panel: DAPI-staining of cell nuclei, providing an overview of the tissue section used for immunostaining
View 4 more images

Applications for Cytokeratin 18 Antibody (RGE53) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:100-1:200

Western Blot

1:100-1:1000
Application Notes
This antibody is suitable for immunoblotting, immunocytochemistry on acetone fixed cells, immunohistochemistry on frozen sections and flow cytometry. Optimal antibody dilution should be determined by titration; recommended range is 1:100 - 1:200 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

This antibody is CyTOF ready..
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS, pH 7.2

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cytokeratin 18

Cytokeratins are cytoskeletal intermediate filament proteins that are classified as type I (acidic) and type II (neutral to basic) and associate into heterotetramers.

Alternate Names

CK-18, CYK18, Keratin 18, KRT18

Gene Symbol

KRT18

Additional Cytokeratin 18 Products

Product Documents for Cytokeratin 18 Antibody (RGE53) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Cytokeratin 18 Antibody (RGE53) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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