Cytokeratin 18 Antibody (SPM265) [Janelia Fluor® 525]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34404JF525
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Bovine (Negative), Canine (Negative), Hamster (Negative), Mouse (Negative), Porcine (Negative), Rat (Negative), Sheep (Negative)
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Janelia Fluor 525
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM265
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Cytokeratin 18 Antibody (SPM265) [Janelia Fluor® 525]
Immunogen
PMC-42 human breast carcinoma cells
Reactivity Notes
Does not react with Mouse, Rat, Sheep, Hamster, Bovine, Canine or Porcine.
Localization
Cytoplasmic
Specificity
This monoclonal antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
Marker
Epithelial Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Applications for Cytokeratin 18 Antibody (SPM265) [Janelia Fluor® 525]
Application
Recommended Usage
CyTOF-ready
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Cytokeratin 18
Alternate Names
CK-18, CYK18, Keratin 18, KRT18
Gene Symbol
KRT18
Additional Cytokeratin 18 Products
Product Documents for Cytokeratin 18 Antibody (SPM265) [Janelia Fluor® 525]
Product Specific Notices for Cytokeratin 18 Antibody (SPM265) [Janelia Fluor® 525]
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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