Cytokeratin 19 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-687
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Rat
Predicted:
Orangutan (98%). Backed by our 100% Guarantee.
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Synthetic peptide derived from the C-terminal region of human Cytokeratin 19 (between residues 350-400) [UniProt P08727]
Reactivity Notes
Rat reactivity reported in scientific literature (PMID: 25401473)
Localization
Cytoplasmic.
Marker
Epithelial Cell Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Cytokeratin 19 Antibody - BSA Free
Simple Western: Cytokeratin 19 AntibodyBSA Free [NB100-687]
Simple Western: Cytokeratin 19 Antibody [NB100-687] - Lane view shows a specific band for Cytokeratin 19 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under standard reducing conditions using the 12-230 kDa separation system.Western Blot: Cytokeratin 19 AntibodyBSA Free [NB100-687]
Western Blot: Cytokeratin 19 Antibody [NB100-687] - Western blot detection of YAP and TAZ in proteins extracted from isolated hepatocytes and cholangiocytes and total liver tissue. Antibodies against CK19 and HNF4a were used to confirm purity of the populations and b-actin was used as a loading control. Representative results from a single experiment with n = 3 independent cell isolations and n = 2 total liver tissues. Image collected and cropped by CiteAb from the following publication(https://embomolmed.embopress.org/lookup/doi/10.15252/emmm.201506089), licensed under a CC-BY license.Immunocytochemistry/ Immunofluorescence: Cytokeratin 19 Antibody - BSA Free [NB100-687]
Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody [NB100-687] - TUDCA induces intermediate states of mesenchymal and epithelial cells in primary cultures of rat HSC during hepatic differentiation. Under control conditions HSC remained negative for K19 (Cytokeratin 19) expression, (d) but this epithelial marker protein was induced in HSC cultures after treatment with 2 uM TUDCA for 14 days (red). (f) K19 was found to be co-expressed with desmin (green), which indicated the origin of epithelial progenitor cells from HSC. (I) Cells with different states of maturation were still found after 21 days of TUDCA treatment. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep13320), licensed under a CC-BY license.Applications for Cytokeratin 19 Antibody - BSA Free
Application
Recommended Usage
Flow Cytometry
2-5 ug/million cells
Immunocytochemistry/ Immunofluorescence
1:50-1:100
Immunohistochemistry
1:500
Immunohistochemistry-Paraffin
1:500
Simple Western
1:1000
Western Blot
1:1000
Application Notes
This Cytokeratin 19 antibody is useful for Western Blot where a band is seen ~44 kDa, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry-paraffin sections. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 3 using NB100-687 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS and 30% Glycerol
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Cytokeratin 19
Alternate Names
CK19, EndoC, K19, Krt19
Gene Symbol
KRT19
UniProt
Additional Cytokeratin 19 Products
Product Documents for Cytokeratin 19 Antibody - BSA Free
Product Specific Notices for Cytokeratin 19 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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