Cytokeratin 2 Antibody (9T3Q2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16139

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16139-100ul
NBP3-16139-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9T3Q2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Cytokeratin 2 Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 293-422 of human KRT2 (P35908). YMIKVELQSKVDLLNQEIEFLKVLYDAEISQIHQSVTDTNVILSMDNSRNLDLDSIIAEVKAQYEEIAQRSKEEAEALYHSKYEELQVTVGRHGDSLKEIKIEISELNRVIQRLQGEIAHVKKQCKNVQD

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cytokeratin 2 Antibody (9T3Q2)

Western Blot: Cytokeratin 2 Antibody (9T3Q2) [NBP3-16139]

Western Blot: Cytokeratin 2 Antibody (9T3Q2) [NBP3-16139]

Western Blot: Cytokeratin 2 Antibody (9T3Q2) [NBP3-16139] - Western blot analysis of extracts of A-431 cells, using Cytokeratin 2 antibody (NBP3-16139) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry: Cytokeratin 2 Antibody (9T3Q2) [NBP3-16139]

Immunohistochemistry: Cytokeratin 2 Antibody (9T3Q2) [NBP3-16139]

Immunohistochemistry: Cytokeratin 2 Antibody (9T3Q2) [NBP3-16139] - Immunofluorescence analysis of mouse skin using Cytokeratin 2 Rabbit mAb (NBP3-16139) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: Cytokeratin 2 Antibody (9T3Q2) [NBP3-16139]

Immunohistochemistry-Paraffin: Cytokeratin 2 Antibody (9T3Q2) [NBP3-16139]

Immunohistochemistry-Paraffin: Cytokeratin 2 Antibody (9T3Q2) [NBP3-16139] - Mouse skin using Cytokeratin 2 Rabbit mAb (NBP3-16139) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Applications for Cytokeratin 2 Antibody (9T3Q2)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cytokeratin 2

Alternate Names

CK-2e, Cytokeratin-2e, epidermal ichthyosis bullosa of Siemens, Epithelial keratin-2e, K2e, keratin 2, keratin 2A (epidermal ichthyosis bullosa of Siemens), keratin, type II cytoskeletal 2 epidermal, Keratin-2 epidermis, Keratin-2e, KRT2AMGC116967, KRT2EMGC116968, KRTE, Type-II keratin Kb2

Gene Symbol

KRT2

Additional Cytokeratin 2 Products

Product Documents for Cytokeratin 2 Antibody (9T3Q2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Cytokeratin 2 Antibody (9T3Q2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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